在表观遗传驱动的修复过程中,在牙髓细胞中表达miRNA的特征
Michaela Kearney1, Paul R Cooper2, Anthony J Smith3
1Division of Restorative Dentistry & Periodontology, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, D02 F859 Dublin, Ireland.
International journal of molecular sciences
|May 27, 2023
概括
下一代生物材料利用表观遗传机制,包括微RNA (miRNA) 和表观遗传修饰剂,以刺激牙纸细胞修复和矿化. 这项研究揭示了miRNAs和表观遗传药物在这个过程中的动态相互作用.
科学领域:
- 复原性内牙科 复原性内牙科
- 生物材料科学 生物材料科学
- 分子生物学分子生物学
背景情况:
- 再生内科医疗旨在开发针对表观遗传机制的先进生物材料,如miRNAs,基因素乙化和DNA甲基化,以修复脉.
- 基因组脱乙酶抑制剂 (HDACi) 和DNA甲基转移酶抑制剂 (DNMTi) 促进牙纤维细胞 (DPC) 的矿化,但它们在这一过程中与miRNAs的相互作用尚不清楚.
研究的目的:
- 在DPC矿化过程中分析miRNA表达.
- 研究HDACi (SAHA) 和DNMTi (5-AZA-CdR) 对miRNA表达,DPC矿化和扩散的影响.
- 在DPC修复过程中阐明miRNAs和表观遗传修饰剂之间的相互作用.
主要方法:
- 小RNA测序和生物信息分析以建立miRNA表达特征.
- 用SAHA和5-AZA-CdR对DPC进行处理,以评估对矿化,增殖和miRNA表达的影响.
- 定量逆转录PCR (qRT-PCR) 用于验证miRNA表达变化.
主要成果:
- 无论是SAHA还是5-AZA-CdR,都增强了DPC矿化,但减少了细胞增殖.
- 表观遗传修饰导致miRNA表达的广泛变化,确定了miRNAs可能调节Wnt和MAPK通路.
- qRT-PCR证实了在DPC矿化过程中对表观遗传抑制剂的反应中选择的miRNAs的差异调节.
结论:
- 表观遗传药物治疗诱导DPC中的矿化,伴随着miRNA表达的显著改变.
- 在DPC修复过程中,miRNAs和表观遗传修饰剂之间存在动态相互作用,为向的再生疗法提供了潜力.
- 这些发现突出了miRNAs在调解DPC矿化和分化的表观遗传影响中的作用.
关键词:
这是一种DNA甲基转移酶抑制剂.有关RNA测序的RNA测序牙髓干细胞是牙髓的干细胞.表观遗传学是指表观遗传学.基因素脱乙酶抑制剂 基因素脱乙酶抑制剂这是一个微型RNA.没有编码的RNA.生命脉治疗 治疗生命脉更多相关视频
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