乙基酸盐通过促进IFITM3表达,溶酶体酸化和蛋白酶活性来抑制牛病毒性腹病毒
Linlin Zhang1, Guanghui Yang1, Jun Wang1
1College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China.
International journal of molecular sciences
|May 27, 2023
概括
乙基酸 (EG) 通过阻断病毒的进入和复制,有效地抑制牛病毒性腹病毒 (BVDV) 在MDBK细胞中的感染. 这种抗病毒活性与IFITM3表达的增加和自的调节有关,这表明EG与IFITM3表达的增加有关.
科学领域:
- 兽医病毒学 兽医病毒学
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 牛病毒性腹病毒 (BVDV) 由于其高度传染性,对畜牧业构成重大经济威胁.
- 乙基酸 (EG),一种酸衍生物,表现出多种生物活性,包括抗氧化和抗菌特性,使其成为调节宿主-病原体相互作用的候选者.
研究的目的:
- 为了研究乙基酸 (EG) 对牛病毒性腹病毒 (BVDV) 的抗病毒作用,在马丁-达比牛 (MDBK) 细胞中.
- 阐明BVDV感染细胞中EG的潜在抗病毒机制.
主要方法:
- 马丁 - 达比牛 (MDBK) 细胞被BVDV感染,并用不同剂量的乙基酸盐 (EG) 治疗.
- 病毒复制,细胞活力,病毒附着,入口,释放,干扰素诱导的跨膜蛋白3 (IFITM3) 表达,甲素B水平,亚克里丁色和自标志物 (LC3,p62) 通过西斑和免疫光测试进行了评估.
- 在调节IFITM3表达过程中,自的作用被用克洛洛昆和拉帕米辛研究.
主要成果:
- 乙基酸盐 (EG) 在非细胞毒性度下显著抑制了MDBK细胞中的BVDV感染,在同时治疗和治疗后都有效.
- 通过阻止病毒的进入和复制,EG抑制了BVDV感染,但没有附着或释放.
- EG治疗上调调节了干扰素诱导的跨膜蛋白3 (IFITM3) 表达和增强的甲素B水平和溶酶体酸化.
- 根据LC3和p62水平的增加,EG治疗促进了自,并且可以通过自调节IFITM3的表达.
结论:
- 乙基酸 (EG) 对牛病毒性腹病毒 (BVDV) 在MDBK细胞中的复制表现出强大的抗病毒活性.
- 抗病毒机制涉及增强的IFITM3表达,改善的淋巴体酸化,增加的蛋白酶活性和调节的自.
- EG显示出作为牛BVDV感染的治疗剂的潜力.
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