用抗TNF药物治疗克罗恩病患者的短期缓解预测模型的开发
Rosario Medina-Medina1, Eva Iglesias-Flores1, Jose M Benítez1
1Gastroenterology Unit, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Hospital Universitario Reina Sofía, Universidad de Córdoba, 14004 Córdoba, Spain.
International journal of molecular sciences
|May 27, 2023
概括
在克罗恩病中确定抗瘤亡因子 (TNF) 治疗的生物标志物.
科学领域:
- 胃肠道学和免疫学
- 蛋白质组学和生物标志物发现发现
背景情况:
- 抗瘤坏死因子 (TNF) 治疗显著影响克罗恩病 (CD) 管理.
- 长期有效性丧失和不良事件需要可靠的反应标志物.
- 预测患者对抗TNF药物的反应对于个性化治疗至关重要.
研究的目的:
- 为了确定预测性蛋白质生物标志物用于短期缓解 (STR) 在抗TNF天真克罗恩病患者.
- 调查与抗TNF治疗反应相关的蛋白质表达特征.
主要方法:
- 根据临床反应分层的113名抗TNF天真CD患者的血样本的SWATH蛋白质组学分析 (12周后的STR与非STR).
- 在响应者和非响应者之间比较治疗前蛋白质表达特征.
- 使用酶相关免疫吸收试验 (ELISA) 验证候选生物标志物.
主要成果:
- 鉴定了18种差异表达的蛋白质 (p ≤0.01,折叠变化≥2.4),与细胞骨,血液静止,新陈代谢和免疫反应有关.
- 温古林成为显著放松的蛋白质 (p < 0.001),其差异性表达得到了ELISA的证实 (p = 0.054).
- 多变量分析表明血温林水平,基底CD活性指数,皮质类固醇使用和先前的肠切除预测非STR.
结论:
- 血蛋白质概况,特别是素,可以作为在克罗恩病中对抗TNF治疗反应的预测生物标志物.
- 这些发现可能有助于优化抗TNF治疗策略和患者选择.
- 需要进一步验证,以将这些生物标志物纳入临床实践.
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