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Ru (II) 氧气传感器用于Co (III) 综合体和安二B抗真菌活性检测通过光光学呼吸计

Katarzyna Turecka¹, Agnieszka Chylewska², Aleksandra M Dąbrowska²

¹Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Gdańsk, al. Hallera 107, 80-416 Gdańsk, Poland.

International journal of molecular sciences

|May 27, 2023

概括

研究人员开发了基于的新型氧气传感器,以评估抗真菌活性. 这些传感器揭示了安二B和可溶性氧传感器之间的协同作用,增强了对Candida albicans的抗真菌功效.

科学领域:

生物化学和生物物理学
材料科学材料科学材料科学
微生物学微生物学

背景情况:

氧气消耗对于理解新陈代谢状态至关重要.
氧气的光灭特性使得氧气传感器的开发成为可能.
基于的复合物是有效的对氧敏感探头.

研究的目的:

通过使用新型氧气传感器,评估 (III) 复合物的抗真菌活性和B氨.
描述一种新的水溶性 (BsOx) 复合物作为氧气传感器.
为了研究抗真菌剂和BsOx传感器之间的潜在协同效应.

主要方法:

使用RP-UHPLC,LCMS,MALDI,NMR和UV-Vis.等技术合成和表征水溶性 (BsOx) 复合物.
基于固定和可溶性Ru (II) 的氧气传感器的开发.
在RPMI和血清中进行微生物学测试,以测试对Candida albicans的抗真菌活性.
用氧气灭光的评估,以监测代谢活性和药物疗效.

主要成果:

基于固定和可溶性Ru (II) 的传感器都有效地研究了Co (III) 复合体和安二乙酸的活性.
可溶性BsOx传感器在与安福特 B.结合时显示出一种新的协同效应.
这种组合显著增强了对Candida albicans的抗真菌活性.
该研究成功地证明了化合物对微生物的协同作用.

结论:

基于的氧气传感器是评估抗真菌药物疗效的宝贵工具.
可溶性BsOx传感器本身具有作为抗真菌剂或作为增强剂的潜力.
在BsOx和B. amphotericin之间观察到显著的协同抗真菌作用.
这项研究为开发针对真菌感染的新型组合疗法开辟了道路.

关键词:

在Candida albicans中使用.复合物中的Co (III) 复合物.抗真菌活动的抗真菌活性.氧气传感器氧气传感器光光学呼吸计光学呼吸计

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