丸微RNA在热应激诱导的精子发生障碍中的潜在功能
Mailin Gan1,2, Yunhong Jing1,2, Zhongwei Xie1,2
1Key Laboratory of Livestock and Poultry Multi-Omics, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, China.
International journal of molecular sciences
|May 27, 2023
概括
丸热应激显著损害了小鼠的精子生产和质量. 微RNA (miRNA) 和mRNA表达变化揭示了潜在的调节途径,涉及热引起的丸缩和精子生成障碍.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 精子生成对温度波动非常敏感.
- 丸温度升高会对哺乳动物的精子生产和精液质量产生负面影响.
研究的目的:
- 调查急性热应激对小鼠丸功能的影响,并确定关键的分子调节剂.
- 在暴露于热量后分析微RNA (miRNA) 和信使RNA (mRNA) 表达的变化.
主要方法:
- 在小鼠中诱导丸热应激,使用43°C的水浴25分钟.
- 对丸重量,精子密度和高通量测序进行评估,用于miRNA和mRNA分析.
- 生物信息学分析包括基因本体学 (GO) 和miRNA-mRNA联合表达网络的构建.
主要成果:
- 热应激导致第7天丸体重 (至68.45%) 和精子密度 (至33.20%) 显著减少.
- 不同表达分析揭示了许多miRNA (98下降,77上升) 和mRNA (369下降,124上升) 的显著变化.
- 基因组分析表明热应激与丸缩和精子生成障碍通过细胞半变异和细胞循环调节有关. 确定了miR-143-3p作为一种潜在的关键调节剂.
结论:
- 热应激严重破坏小鼠的精子生成和精液质量.
- 调节细胞半分裂和细胞循环的分子通路与热引起的丸功能障碍有关.
- miR-143-3p成为缓解热应激诱导的生殖问题的潜在治疗标.
关键词:
热应激是一种热应激.不孕不育 不孕不育 不孕不育微RNAs 是一个微型RNA.精子发生障碍 精子发生障碍丸缩 testicular atrophy testicular atrophy testicular atrophy testicular atrophy testicular atrophy testicular atrophy testicular atrophy testicular atrophy testicular atrophy 丸缩 testicular atrophy testicular atrophy testicular atrophy testicular atrophy testicular atrophy 丸缩 testicular atrophy testicular atrophy testicular atrophy testicular atrophy testicular atrophy testicular atrophy 丸缩 testicular atrophy 丸缩 testicular atrophy testicular atrophy testicular atrophy testicular atrophy testicular atrophy
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