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多omics数据集成揭示了在高血糖微观环境中导致下微生物组失生症引起的炎症反应的关键变量

Sarah Lafleur¹, Antoine Bodein², Joanna Mbuya Malaïka Mutombo¹

¹Oral Ecology Research Group (GREB), Faculty of Dentistry, Université Laval, 2420 rue de la Terrasse, Québec, QC G1V 0A6, Canada.

International journal of molecular sciences

|May 27, 2023

概括

在高葡萄糖环境下下微生物组失生症通过增加炎症而加剧牙周炎症. 关键的基因,细胞因子和细菌被确定为这种宿主微生物群相互作用的关键因素.

科学领域:

口腔生物学口腔生物学
微生物学微生物学
免疫学免疫学免疫学

背景情况:

下微生物组失调是牙周炎的关键驱动因素,牙周炎是一种与代谢疾病相关的慢性炎症状况.
过高血糖微环境对宿主微生物群相互作用和牙周炎炎炎症的影响仍然未得到充分研究.

研究的目的:

为了研究高血糖微环境对炎症反应和基因表达的影响,在一个由异菌性子菌微生物刺激的牙培养模型中.
在高血糖条件下确定关键的分子和微生物参与牙周炎症的参与者.

主要方法:

一个牙共养模型 (HGF-1和U937细胞) 用来自健康捐赠者和牙周炎患者的牙下微生物组来刺激.
测量了炎症标志物 (细胞因子,MMPs),并对共同培养进行了转录组分析 (微阵列).
使用16SrRNA基因测序分析了下微生物组,并使用多omics方法整合了数据.

主要成果:

特定的基因 (krt76, krt27, pnma5, mansc4, rab41, thoc6, tm6sf2, znf506) 被确定为关键变量.
促炎性细胞因子 (IL-1β,GM-CSF,FGF2,IL-10) 和矩阵金属蛋白酶 (MMP3,MMP8) 发生了显著的改变.
特定的细菌ASVs和属 (Prevotella,Campylobacter,Fretibacterium) 与炎症反应相关.

结论:

多omics集成揭示了复杂的相互关系,在高血糖微环境中调节牙周炎症.
该研究强调了在高葡萄糖条件下导致牙周炎病原发生的特定宿主和微生物因素.

关键词:

计算生物学是计算生物学.细胞因子细胞因子这种疾病叫做dysbiosis.过高血糖症 (hyperglycemia) 是一种这是一种炎症炎症炎症炎症.这就是metagenomic的意义.金属蛋白酶是一种金属蛋白酶.微生物组是一个微生物组.奥米克斯集成技术的整合.牙周炎是指牙周炎的一种疾病.

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