异位表达PsnNAC090增强了转基因烟草中的盐和奥斯摩斯耐受性
Yuting Wang1, Wenjing Zang1, Xin Li1
1State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
International journal of molecular sciences
|May 27, 2023
概括
在植物中,NAC转录因子PsnNAC090增强了盐和透耐受性. 该基因通过促进活性氧物种的清理和减少转基因烟草中的膜损伤来改善应激反应.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 遗传学 遗传学 是一个
背景情况:
- NAC转录因子对于植物发育和应激适应至关重要.
- 可诱导盐的NAC基因PsnNAC090从Populus simonii × Populus nigra中分离出来.
研究的目的:
- 描述PsnNAC090基因并研究其在植物耐压力中的作用.
- 分析蛋白质的定位,转录活性和DNA结合特性.
主要方法:
- 基因隔离和促进体分析.
- 在烟草和洋表皮细胞中进行过渡性表达分析.
- 酵母的一杂种和两杂种测试.
- 在盐和透应激下对基因表达的分析.
- 转基因烟草的生成和生理分析.
主要成果:
- PsnNAC090蛋白定位到细胞核,细胞质和细胞膜,具有转录激活活性.
- 该基因与ABA响应元素 (ABREs) 结合,在压力下的根系中高度表达.
- 在烟草中过度表达PsnNAC090增加了对盐和透应激的耐受性.
结论:
- PsnNAC090在改善植物对盐和透应激的耐受性方面发挥着重要作用.
- 该基因通过改善反应性氧物种清理和减少膜脂质过氧化来增强应激耐受性.
- PsnNAC090是开发抗压作物的有希望的候选基因.
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