三酸盐道金属酶2在ABA介导的种子发芽中作为ABI4的下游因子
Yu-Rui Feng1, Ting-Ting Li2, Shi-Jia Wang1
1State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.
International journal of molecular sciences
|May 27, 2023
概括
这项研究表明,TTM2对于酸 (ABA) 介导的种子发芽至关重要. ABA抑制了TTM2的表达,这是种子正常发育和发芽所需的.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 种子发芽是一个关键的发育过程,受酸 (ABA) 的影响.
- 三酸盐道金属酶 (TTM) 超级酶家族的生物学作用仍然在很大程度上未被探索.
- TTM2是TTM超级家族的成员,在植物发育中具有未知的功能.
研究的目的:
- 研究TTM2在酸 (ABA) 介导的种子发芽和幼苗早期发育中的功能.
- 阐明ABA对TTM2的监管机制及其上游因素.
- 确定TTM2在ABA信号通路中的作用.
主要方法:
- 在种子发芽期间在ABA治疗下分析TTM2表达模式.
- 使用TTM2淘汰突变体和过度表达线的基因分析.
- 研究ABA无敏4 (ABI4) 和TTM2促进体之间的相互作用.
- 野生型,突变型和转基因植物的表型分析.
主要成果:
- 在种子发芽过程中,ABA既增强了TTM2的表达,又抑制了它的表达.
- 过度表达TTM2可以挽救ABA诱导的种子发芽和幼苗发育的抑制.
- ttm2突变显示了发芽率的降低和叶绿化,这表明TTM2需要ABA介导的抑制.
- ABA通过ABI4与TTM2促进体结合来抑制TTM2表达,将TTM2定位在ABI4.4的下游.
结论:
- 在ABA介导的种子发芽和幼苗早期发育中,TTM2起着至关重要的作用.
- 通过ABA抑制TTM2表达,由ABI4调解,对于调节这些过程至关重要.
- 在 ABA 信号通路中,TTM2 作为 ABI4 的下游组件.
关键词:
阿巴阿巴阿巴是什么意思ABI4 ABI4 ABI4 ABI4 ABI4 ABI4 ABI4 ABI4 ABI4 ABI4 ABI4 ABI4 ABI4 ABI4在TTM2中,种子的发芽 种子的发芽更多相关视频
08:52A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana
Published on: November 9, 2013
13.6K
08:31Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
Published on: March 29, 2019
29.6K
相关概念视频
01:45Tail-anchoring of Proteins in the ER Membrane
3.1K
Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
3.1K
01:19Allosteric Proteins-ATCase
5.8K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.8K