消耗ATP与排泄的中性粒细胞内核细胞分裂相关
Duo Wang1, Zirui Zeng2, Mengyue Shen3,4
1Department of Radiobiology and Hygiene Management, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
International journal of molecular sciences
|May 27, 2023
概括
中性粒细胞内细胞分裂依赖于腺三酸盐 (ATP) 的能量代谢. 抑制特定的酶和途径会影响该过程中的ATP消耗,揭示关键的分子事件.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物化学 生化学
背景情况:
- 中性细胞化涉及能量代谢,而腺三酸盐 (ATP) 对内细胞化至关重要.
- 之前的工作建立了一种流细胞计系统,用于测量颗粒物中性粒细胞内细胞化.
研究的目的:
- 为了研究中性粒细胞内细胞分裂和细胞能量消耗之间的复杂关系.
- 阐明在中性粒细胞内细胞分裂过程中控制ATP利用的分子机制.
主要方法:
- 利用流细胞计系统来测量中性粒细胞的颗粒物内细胞化.
- 采用了动氨酸抑制剂,外源ATP和ATP合成酶的抑制剂,尼古丁胺氨基二核酸酸盐氧化酶,酸氨基醇-3激酶和I kappa B激酶 (IKK).
- 通过使用缺少NLR家族pyrin域的淘汰小鼠检查了中性粒细胞的行为,其中包含三个.
主要成果:
- 动氨酸抑制抑制了中性粒细胞内细胞分裂期间的ATP消耗.
- 中性粒细胞的内细胞行为因外源ATP度而异.
- 抑制ATP合成酶和尼古丁胺氨酸二核酸盐氧化酶,但不抑制酸氨基醇-3激酶,抑制了内细胞分裂.
- 核因子kappa B在内细胞分裂期间的激活被IKK抑制剂逆转,这也恢复了ATP消耗.
- 炎症酶激活与中性粒细胞内细胞分裂或ATP消耗无关.
结论:
- 中性粒细胞内细胞分裂与以ATP为中心的能量代谢密切相关.
- 特定的分子通路,包括动氨酸,ATP合成酶和尼古丁胺氨基二核酸氧化酶,对于调节内细胞分裂期间的能量消耗至关重要.
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