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在COVID-19期间,CD56^-和CD56⁺ T细胞子组的变化

Julia D Vavilova¹, Maria O Ustiuzhanina^1,2, Anna A Boyko¹

¹Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.

International journal of molecular sciences

|May 27, 2023

概括

自然杀手T (NKT) 类细胞在COVID-19期间在抗病毒免疫力中发挥着至关重要的作用. 在严重病例中,它们的激活和分化发生了改变,这表明它们在对抗病毒方面起着保护作用.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
细胞生物学细胞生物学

背景情况:

细胞毒性T细胞激活对于抗病毒免疫反应至关重要.
自然杀手T (NKT) 样细胞,是一种表达CD56的T淋巴细胞的子集,具有结合T细胞和NK细胞的特性.
在COVID-19病变发生过程中,NKT类细胞的作用尚不完全理解.

研究的目的:

研究COVID-19患者中循环NKT样细胞和CD56-T细胞的激活和分化.
为了在不同COVID-19严重程度组 (ICU,中度严重程度) 和康复者中比较这些免疫细胞子集.
为了确定潜在的生物标志物,表明COVID-19的进展.

主要方法:

对外围血液单核细胞的流细胞计分析.
量化T细胞子集,包括CD56+ (NKT类) 和CD56-T细胞.
细胞表面标记物表达的评估 (例如,CD8,KIR2DL2/3,NKp30,NKG2D,NKG2A,PD-1,HLA-DR,CD16). 细胞表面标记物表达的评估包括:

主要成果:

在重症监护室 (ICU) 患者中观察到CD56+ T细胞的比例降低,结果致命.
严重的COVID-19显示CD8+T细胞减少,主要是由于CD56细胞死亡,以及转向NKT类子集中的更差异化的细胞毒性CD8+T细胞.
在COVID-19患者和康复者CD56+T细胞中发现KIR2DL2/3+和NKp30+细胞的比例增加,而在CD56-和CD56+T细胞中发现NKG2D+和NKG2A+细胞的减少和PD-1和HLA-DR表达的增加.
中度重症和致命ICU患者的CD56-T细胞中CD16水平升高表明CD56-CD16+T细胞具有有害作用.

结论:

CD56+ T细胞在COVID-19中表现出抗病毒作用.
T细胞子集的变化,特别是CD56+ T细胞,与COVID-19的严重程度和结果有关.
特定的细胞表面标记物 (PD-1,HLA-DR,CD16) 可能作为疾病进展的指标.

关键词:

CD56+ T 细胞为CD56+ T 细胞在 COVID-19 疫情中,在 HLA-DR 组合下,KIR2DL2/3/3 的情况.类似NKT细胞的细胞.在 NKp30 中,NKp30 是 NKp30 的代码.这就是PD1 PD1.在T细胞,T细胞.

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