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尿素通过诱导铁灭菌来抑制肝细胞癌细胞的发展

Liuliu Zi^1,2, Wangbin Ma¹, Lilong Zhang¹

¹Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, No. 238, Jiefang Road, Wuchang District, Wuhan 430060, China.

Journal of clinical medicine

|May 27, 2023

概括

尿液水平在肝细胞癌 (HCC) 组织中发生变化. 补充尿素通过激活ferroptosis来抑制HCC细胞的增殖和迁移,这表明其具有治疗潜力.

科学领域:

生物化学生物化学
在瘤学瘤学.
代谢学代谢学代谢学

背景情况:

尿素是DNA,RNA和葡萄糖合成的重要代谢物,主要在肝脏中产生.
尿素在肝细胞癌 (HCC) 瘤微环境中的作用及其治疗潜力仍然未被探索.
改变尿素代谢可能有助于HCC的发展和进展.

研究的目的:

为了研究HCC组织中的尿素水平.
探索尿素在HCC中的治疗疗效.
阐明尿素抗癌作用的潜在机制.

主要方法:

在HCC组织中使用组织微阵列 (n=115) 进行de novo尿素合成酶 (CAD,DHODH) 的基因表达分析.
通过LC-MS/MS (n=46) 来量化HCC和相邻非瘤组织中的尿素含量.
在体外和体内实验中评估尿素对HCC细胞增殖,入侵和迁移的影响.

主要成果:

在HCC组织中观察到CAD和DHODH的表达升高,而不是瘤组织.
在HCC组织中检测到明显较低的尿素含量 (484.22nmol/g) 与非瘤组织 (640.36nmol/g) 相比.
尿素的剂量依赖,通过激活铁亡途径来抑制HCC细胞的增殖,入侵和迁移.

结论:

在HCC中尿液代谢受到干扰,瘤组织中的水平降低.
尿素通过诱导铁亡来表现出对HCC的抗癌特性.
尿素代表了肝细胞癌的一个有前途的新型治疗标.

关键词:

在这里,我们可以看到CAD,CAD,CAD.DHODH DHODH 在线阅读铁性化 (ferroptosis) 是一种肝细胞癌是肝细胞癌.瘤微环境是一个微环境.尿素尿素尿素

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