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相关概念视频

Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

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相关实验视频

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Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
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基于流体控制的高效单细胞封闭微器件

Daiki Tanaka1, Junichi Ishihara2, Hiroki Takahashi2,3,4

  • 1Research Organization for Nano & Life Innovation, Waseda University, 513 Waseda Tsurumakicho, Shinjuku-ku, Tokyo 162-0041, Japan.

Micromachines
|May 27, 2023
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概括

研究人员开发了一种新的形微流体装置,用于有效地捕捉和培养单个细菌. 这种新设备显著提高了储存效率,并允许快速化学交换,有利于对耐药细菌的研究.

关键词:
细胞培养培养的细胞培养.流体控制器的流体控制器微流体设备的微流体设备一个单细胞的单细胞.

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相关实验视频

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科学领域:

  • 微流体学 微流体学
  • 细胞生物学 细胞生物学
  • 细菌学 细菌学是一门学科.

背景情况:

  • 传统的方法难以捕获单个细菌,需要离心.
  • 现有的设备在有效的细胞存储和快速化学操纵方面存在挑战.
  • 培养耐药细菌需要先进的微流体溶液.

研究的目的:

  • 开发一种高效的微流体装置,用于捕获和培养单个细菌.
  • 提高储存效率,并为细菌研究提供快速化学交换.
  • 研究微流体生长通道内的压力动态.

主要方法:

  • 使用软微电子机械系统 (MEMS) 制造一个形的微流体装置.
  • 利用流动液体在生长通道内储存细菌.
  • 使用计算模拟来分析生长通道中的压力损失.

主要成果:

  • 使用微珠实现了储存效率从0.2%到84%的显著提高.
  • 与传统设备 (>1400 PaG) 相比,在生长通道中减轻了压力损失 (<400 PaG).
  • 成功地将该设备应用于培养各种细菌,包括*Salmonella enterica*和*Staphylococcus aureus*.

结论:

  • 开发的微流体装置为单细胞捕获和培养提供了卓越的性能.
  • 它的快速化学交换能力是研究耐药细菌菌株的理想选择.
  • 该装置的多功能性和易于制造使其在微生物学研究中广泛适用.