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疫苗后G8P的演变[4]在卢旺达的一个罗塔病毒菌株组;在中和位的显著变异

Peter N Mwangi¹, Robyn-Lee Potgieter¹, Jeannine Uwimana²

¹Next Generation Sequencing Unit, Division of Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein 9300, South Africa.

Pathogens (Basel, Switzerland)

|May 27, 2023

概括

非洲轮状病毒菌株,特别是G8P[4],具有很高的遗传多样性,可能会影响疫苗的有效性. 卢旺达菌株的全基因组测序揭示了独特的进化路径和牛基因的重组,这表明持续监测至关重要.

科学领域:

病毒学病毒学
遗传学是一个遗传学.
流行病学流行病学

背景情况:

非洲在轮状病毒菌株中表现出显著的遗传多样性,这可能解释了轮状病毒疫苗有效性的降低.
在非洲地区,G8P[4]菌株是这种轮状病毒多样性的显著贡献者.

研究的目的:

为了充分阐明在卢旺达发现的G8P[4]轮状病毒菌株的基因组和进化史.
调查潜在的遗传因素,有助于非洲轮状病毒菌株的疫苗规避.

主要方法:

使用Illumina技术对21个卢旺达G8P[4]轮状病毒菌株进行了全基因组测序.
生物遗传学分析被用来确定测序菌株与已知的人类和动物轮状病毒菌株的进化关系.

主要成果:

在卢旺达的21个G8P[4]菌株中,有20个具有类似DS-1的基因型星座,其中一个具有重组基因型.
与疫苗菌株相比,在中和部位发现了显著的氨基酸差异,表明潜在的中和逃脱.
遗传学分析将五个基因组段与东非人类轮回病毒菌株联系起来,两个NSP4段与牛菌株联系起来,VP1/VP3序列与RotaTeqTM疫苗WC3牛基因联系起来.

结论:

卢旺达G8P[4]轮状病毒菌株的进化,特别是VP1和VP3细分,可能涉及与RotaTeqTM疫苗中的牛基因进行重组.
与肯尼亚和乌干达的G8P[4]菌株有密切的基因关系,这表明它们在东非的共同循环.
持续的全基因组监测对于理解G8P[4]菌株的演变至关重要,特别是后罗塔病毒疫苗的引入.

关键词:

在 G8P[4][4] 中.卢旺达卢旺达卢旺达卢旺达卢旺达卢旺达卢旺达卢旺达卢旺达轮状病毒病毒的病毒.轮状病毒的重新分类.进行全基因组测序.

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