生物发光病毒性Aeromonas hydrophila的发展,以了解病原性
Eda Ozdemir1, Hossam Abdelhamed1, Ozan Ozdemir1
1Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Starkville, MS 39762, USA.
Pathogens (Basel, Switzerland)
|May 27, 2023
概括
这项研究开发了生物发光的Aeromonas hydrophila (vAh),用于追踪道鱼的感染. 研究结果显示,皮肤破裂和作为vAh的关键入口点,导致迅速的全身疾病.
科学领域:
- 水生动物卫生 水生动物卫生
- 微生物学 微生物学
- 鱼类病理学 鱼类病理学
背景情况:
- 由毒性Aeromonas hydrophila (vAh) 引起的移动性Aeromonas败血症 (MAS) 是养殖道鱼的一个重大经济问题.
- 人们对鱼中vAh的感染途径和病原性尚不清楚,这阻碍了有效的疾病管理.
研究的目的:
- 开发一种vAh (BvAh) 的生物发光菌株,用于可视化道鱼的感染动态.
- 通过生物发光成像 (BLI) 研究vAh感染的途径及其在道鱼中的致病性.
主要方法:
- 构建一种新的生物发光表达等离子体 (pAKgfplux3) 并将其引入vAh菌株ML09-119中以创建BvAh.
- 优化稳定的生物发光表达条件,包括氨基醇度和等离子体稳定性.
- 在鱼中使用腹腔内注射,浸泡和修改浸泡 (脂肪片剪切) 的挑战实验,其次是BLI来跟踪BvAh传播.
主要成果:
- 在BvAh中稳定的生物发光表达得到了5-10微克/毫升的氨基醇,尽管它略有减少了生长.
- 没有氨基醇 (半衰期为16小时),BvAh失去了等离子体稳定性.
- BLI证明,MAS通过腹腔内注射进展最快,其次是修改的浸泡和浸泡路线. 检测到BvAh是在前嘴,,底,上皮,受伤的皮肤和上,这表明这些是潜在的入口地点.
结论:
- 皮肤破裂和作为vAh的关键入口点,促进道鱼的快速全身感染.
- 开发的BvAh和BLI技术为vAh-鱼相互作用和病原性提供了有价值的视觉证据.
- 这项研究提高了对通道鱼的vAh毒性机制和感染途径的理解,有助于未来的疾病控制策略.
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