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在受囚禁的甲 (Nymphicus hollandicus) 中发现的Cryptosporidium proventriculi

Mariele Fernanda da Cruz Panegossi¹, Giovanni Widmer², Walter Bertequini Nagata³

¹Faculdade de Medicina Veterinária, Universidade Estadual Paulista (Unesp), Araçatuba 16050-680, São Paulo, Brazil.

Pathogens (Basel, Switzerland)

|May 27, 2023

概括

在巴西,Cryptosporidium parvum被检测到9%的物尾. 胃肠道问题与感染有关,凸显了在照顾鸟类方面需要提高认识的必要性.

科学领域:

兽医寄生虫学兽医寄生虫学
鸟类健康鸟类健康
动物传播疾病动物传播疾病

背景情况:

(Nymphicus hollandicus) 是一个受欢迎的物鸟类.
克里普托斯波里迪亚 spp. 可以导致鸟类的胃肠道疾病.
关于物尾中Cryptosporidium的流行情况的数据有限.

研究的目的:

为了确定Cryptosporidium在家养的流行率.
为了确定与尾中Cryptosporidium感染相关的风险因素.
为了描述影响尾的Cryptosporidium物种.

主要方法:

来自巴西100只家养的便样本.
嵌套PCR针对用于检测的18SrRNA基因.
采用了马六合绿色和修改的金云染色方法.
通过问卷收集有关鸟类养殖和护理的数据.
多变量逻辑回归分析了风险因素.
为了物种识别,PCR amplicons 进行了测序.

主要成果:

对于Cryptosporidium spp. 的流行情况. 通过嵌套的PCR.是9.00%.
染色方法显示患病率较低 (6.00%至7.00%).
胃肠道变化是一个显著的风险因素 (p < 0.01).
测序证实在五个样本中与Cryptosporidium proventriculi有100%的相似性.

结论:

克里普托斯波里迪乌姆 (Cryptosporidium proventriculi) 在被囚禁的中发生.
胃肠道问题与这种物种的Cryptosporidium感染有关.
需要进一步的研究来了解物鸟类的传播和病原性.

关键词:

18S rRNA 基因是18S 的基因.鸟类鸟类鸟类鸟类鸟类这种疾病叫做cryptosporidiosis.流行病学流行病学分子特征的分子表征.患病率的流行情况.有关风险因素的风险因素.

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