相关实验视频

Updated: Jul 29, 2025

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

Published on: November 27, 2014

中性粒细胞参与与BVDV感染相关的免疫功能障碍

Karim Abdelsalam^1,2, Radhey S Kaushik³, Christopher Chase²

¹Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.

Pathogens (Basel, Switzerland)

|May 27, 2023

概括

牛病毒性腹病毒 (BVDV) 通过巨细胞分泌的因素损害中性粒细胞功能. 细胞病变性BVDV菌株特别减少氧化和中性粒细胞外细胞陷的形成,影响牛的免疫力.

科学领域:

兽医免疫学兽医免疫学
病毒学病毒学
细胞免疫学细胞免疫学

背景情况:

牛病毒性腹病毒 (BVDV) 引起免疫功能障碍,导致牛的二次细菌感染.
在BVDV引起的免疫抑制背后的确切机制仍然不清楚.
巨细胞在免疫反应中起着至关重要的作用,并且可以被BVDV感染.

研究的目的:

调查BVDV感染的巨细胞在免疫功能障碍中分泌的因素的作用.
为了确定这些分泌的因素如何影响中性粒细胞的功能.
根据BVDV生物型区分影响.

主要方法:

从BVDV感染的单细胞衍生巨细胞 (MDMs) 中收集了超级活体.
评估了中性粒细胞的功能,包括L-选择素和CD18表达,细胞化,氧化突发,氧化氧化物产生和中性粒细胞外细胞陷 (NET) 诱导.
在非细胞病变 (np) 和细胞病变 (cp) BVDV菌株之间比较了影响.

主要成果:

感染BVDV的MDM超级生物降低了中性粒细胞L-选择蛋白和CD18的表达.
细胞活动和氧化突发被cp和np BVDV 感染的MDMs的超体减少.
氧化的产生和NET的诱导被来自cp BVDV感染的MDMs的超剂特别降低.

结论:

由BVDV感染的巨细胞分泌的因素有助于中性粒细胞免疫功能障碍.
对中性粒细胞的免疫抑制作用,特别是对于氧化和NETs,是BVDV的细胞病变生物型的特征.
这一发现很重要,因为大多数改性活体BVDV疫苗都使用了细胞病变菌株.

关键词:

在 BVDVV 的位置上.这种疫苗是BVDV疫苗.免疫功能障碍免疫功能障碍间接影响间接影响巨细胞是一个巨细胞.作为一个中性恋者.

更多相关视频

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

Published on: June 22, 2016

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

Published on: July 10, 2013

相关概念视频

Immune Response Against Viral Pathogens

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...

B Cell Activation and Differentiation

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...