在体外和体外Ulvan Polysaccharides的Ulva Rigid的作用
Jorge García-Márquez1, Bruna Rodrigues Moreira2, Piedad Valverde-Guillén3
1Department of Microbiology, Faculty of Science, Andalusian Institute of Blue Biotechnology and Development (IBYDA), Malaga University, Campus Universitario de Teatinos s/n, 29071 Malaga, Spain.
Pharmaceuticals (Basel, Switzerland)
|May 27, 2023
概括
来自Ulva rigida的Ulvan在体外显示了对结肠癌细胞的抗癌潜力. 然而,对斑马鱼胚胎的体内研究表明,在较高度下有毒性,这表明治疗用途需要谨慎剂量.
科学领域:
- 海洋生物技术 海洋生物技术
- 药理学 药理学是指药理学的学科.
- 癌症研究 癌症研究
背景情况:
- 乌尔瓦 (Ulvan) 是一种来自乌尔瓦 (Ulva) 品种的硫酸多糖,具有抗癌特性.
- Ulva rigida是一种海洋藻类,是Ulvan的来源.
- 研究ulvan的细胞毒性作用对于其治疗潜力至关重要.
研究的目的:
- 为了评估Ulva rigida的ulvan在体外细胞毒性活动对人类癌症细胞系.
- 评估ulvan对斑马鱼胚胎的体内影响.
- 确定乌尔万作为抗癌剂的潜力,特别是对于结肠癌.
主要方法:
- 在体外细胞毒性测试使用人类癌细胞系 (HCT-116,U-937,G-361) 和健康细胞系 (1064sk,HACAT).
- 使用斑马鱼胚胎进行体内毒性评估.
- 在体外和体外模型中确定致命度50 (LC50) 值.
主要成果:
- 乌尔万在测试的癌细胞系上表现出细胞毒性作用,HCT-116结肠癌细胞具有显著的敏感性 (LC50 = 0.1 mg mL-1).
- 在斑马鱼胚胎的体内研究显示,子度和生长迟缓 (7.8 hpf mL mg-1) 之间存在线性相关性,受精后48小时的LC50约为5.2 mg mL-1.
- 在体内观察到的有毒效应包括心周周 edem 和 LC50 度附近的胆管溶解.
结论:
- 从Ulva rigida提取的Ulvan根据体外结果显示,它是人类结肠癌的潜在治疗剂.
- 在体内斑马鱼胚胎研究表明,乌尔万作为治疗化合物的安全性取决于度,在特定值以上观察到不良影响.
- 乌尔万的治疗应用需要仔细考虑剂量,以减轻潜在的毒性并确保安全,特别是限制度低于0.001 mg mL-1.1.
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