电离辐射对蛋白质或酶溶液中自发形成的聚合物的影响
Karolina Radomska1, Marian Wolszczak1
1Institute of Applied Radiation Chemistry, Faculty of Chemistry, Lodz University of Technology, 93-590 Lodz, Poland.
Pharmaceutics
|May 27, 2023
概括
许多蛋白质和酶形成可逆聚合物. 在氧化应激下的辐射产生稳定,共结合的蛋白质聚合物,主要是二元体,通过涉及氨酸和其他氨基酸的激进反应.
科学领域:
- 生物化学 生物化学
- 化学物理 化学物理
- 材料科学 材料科学 材料科学
背景情况:
- 蛋白质和酶表现出度依赖的可逆聚合.
- 在氧化应激下的辐射会诱导稳定的可溶性蛋白质聚合物的形成.
- 蛋白质二元被假设为主要的聚合结构.
研究的目的:
- 研究N3•和OH基的早期蛋白质氧化.
- 描述辐射诱导蛋白质聚合物的结构和形成机制.
- 评估用于识别辐射产生的蛋白质纳米结构的光谱方法.
主要方法:
- 脉冲放射溶解用于研究与蛋白质的激进反应.
- 静态光谱学 (发射,吸收) 用于总体表征.
- 动态光散射和共振光散射 (RLS) 用于总体检测和分析.
主要成果:
- N3• 激素通过对氨酸残留物之间的共价键形成聚合物.
- •OH激素诱导邻近的蛋白质分子之间各种共价交叉链 (C-C,C-O-C).
- 从氨酸到Trp•基的分子内电子转移会影响聚合物的形成.
- 自发聚合使辐射诱导的纳米结构的识别变得复杂.
- 共振光散射 (RLS) 是检测蛋白质聚合物的敏感方法.
结论:
- 在氧化应激下对蛋白质的辐射会导致由共价键稳定稳定的稳定聚合物.
- 特定的根基反应决定了共价交叉连接和聚合物结构的类型.
- 对于特定的蛋白质系统,标准方法,如dityrosyl交叉链路检测可能需要调整.
- 光化学生命周期测量和RLS对于对辐射产生的蛋白质聚合物的特征有价值.
相关概念视频
01:30Spontaneous and Induced Mutations
47
Spontaneous mutations arise infrequently during DNA replication due to errors in the process. A key factor behind these errors is tautomeric shifts in nitrogenous bases, where bases transition from keto to enol forms or amino to imino forms. This shift can alter base-pairing rules, leading to mutations. Additionally, reactive oxygen species (ROS) arising from aerobic metabolism can damage DNA, resulting in depurination (loss of a purine base) or depyrimidination (loss of a pyrimidine base).
47
01:28Protein Denaturation
4.4K
The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...
4.4K
02:59Biological Effects of Radiation
15.6K
All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
15.6K
01:35Mutations
38.3K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
38.3K