基于酸盐共聚物的热可逆凝作为"智能"药物输送系统,具有长时间的作用,用于肌肉内注射
Igor D Zlotnikov1, Stanislav M Malashkeevich1, Natalia G Belogurova1
1Faculty of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, 119991 Moscow, Russia.
Pharmaceutics
|May 27, 2023
概括
新型热敏凝,包括PEG-基托,可延长药物释放时间长达30天. 这些可注射的多聚合物形成 DNA 多复合体,用于先进的基因传递系统和治疗配方.
科学领域:
- 生物材料科学 生物材料科学
- 聚合物化学 聚合物化学
- 基因传递系统是基因传递系统.
背景情况:
- 热敏凝具有控制药物释放和基因传递的潜力.
- 多重化对于形成DNA多重复的过程至关重要,对基因治疗至关重要.
- 现有的配方缺乏延长释放和有效的基因传递机制.
研究的目的:
- 开发和描述用于DNA多重复形成的新型热敏聚化凝.
- 评估这些凝在延长药物释放 (长达30天) 的潜力.
- 研究这些聚合物在基因传递系统中的应用.
主要方法:
- 热敏共聚合物的合成和表征 (PEG-基托,基托-聚乙烯胺,基托-氨酸,糖醇-基托-精子胺).
- 使用FTIR,UV-vis和光光谱学 (罗达胺6G,阿克里丁色) 进行多重复形成的物理化学分析.
- 评估药物释放动力学 (BSA模型) 和凝降解概况.
- 流细胞测量首次用于研究DNA复合体.
主要成果:
- 同聚合物形成了稳定的DNA复合体,在N/P比为1.0的情况下具有中和DNA电荷.
- 热敏凝,特别是基化,表现出热可逆性质.
- 长时间释放BSA (模型药物) 长达20天,并观察到显著的凝降解.
- 流细胞测量证实了DNA复合体中光粒子的存在.
结论:
- 功能性刺激敏感聚合物是有效的创建长时间的治疗配方.
- 发达的多复合体显示出具有可控制稳定的基因传递载体的潜力.
- 该研究为设计先进的基因传递系统提供了一个平台.
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