Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A Versatile Method for Creating Ultrathin Films of Polyzwitterions with Antifouling Properties.

ACS applied materials & interfaces·2026
Same author

Applications of Isothermal Titration Calorimetry in Studying Biomimetic Nanocarriers.

Biomolecules·2025
Same author

Investigating the Molecular-Level Influence of Surfactants on Polyamide Reverse Osmosis Membrane Formation.

ACS applied materials & interfaces·2025
Same author

Comparative Analysis of Anti-receptor Binding Domain (RBD) IgG Responses to Homologous and Heterologous SARS-CoV-2 Vaccine Regimens: A Study From Bangladesh.

Cureus·2025
Same author

Nanoparticle Tracking Analysis: An Effective Tool to Characterize Extracellular Vesicles.

Molecules (Basel, Switzerland)·2024
Same author

Roadmap on magnetic nanoparticles in nanomedicine.

Nanotechnology·2024

相关实验视频

Updated: Jul 29, 2025

Purification of High Yield Extracellular Vesicle Preparations Away from Virus
07:15

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

11.5K

制备纳米粒子载荷的细胞外囊泡使用直接流过方法.

Shomit Mansur1, Shahriar Habib1, Mikayla Hawkins1

  • 1Chemical & Biological Engineering, The University of Alabama, Tuscaloosa, AL 35487, USA.

Pharmaceutics
|May 27, 2023
PubMed
概括

研究人员开发了一种可扩展的方法,使用直流过来从乳腺癌细胞中产生素-铁纳米粒子载荷的细胞外囊泡 (EV). 这些工程EV显示了增强的细胞吸收,表明改善了药物递送潜力.

关键词:
生物仿真纳米载体 生物仿真纳米载体细胞吸收细胞吸收.直接流过器的直接流过器.药物输送是药物输送的过程.细胞外囊泡蛋白质标记物细胞外囊泡蛋白质标记物用纳米粒子装载的电动汽车.

更多相关视频

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
08:32

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Published on: February 12, 2019

13.8K
Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

4.8K

相关实验视频

Last Updated: Jul 29, 2025

Purification of High Yield Extracellular Vesicle Preparations Away from Virus
07:15

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

11.5K
Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
08:32

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Published on: February 12, 2019

13.8K
Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

4.8K

科学领域:

  • 生物医学工程 生物医学工程
  • 纳米技术纳米技术
  • 癌症治疗方法 癌症治疗方法

背景情况:

  • 细胞外囊泡 (EVs) 显示出作为无细胞疗法和药物输送纳米载体的前景.
  • 可扩展的生产和体内追踪EV仍然是其临床应用的重大挑战.

研究的目的:

  • 开发一种可扩展的方法,使用直流过方法生产纳米粒子装载电动汽车.
  • 与自由纳米粒子相比,特征化纳米粒子载荷的EV并评估它们的细胞吸收.

主要方法:

  • 从MDA-MB-231br癌细胞中通过直接流制备素-铁复合物纳米粒子载荷的EV.
  • 使用传输电子显微镜,动态光散射,SDS-PAGE和EV标记物的抗体阵列进行表征.
  • 使用铁染色的MDA-MB-231br细胞中纳米粒子载荷EVs与自由纳米粒子的细胞吸收的比较.

主要成果:

  • 与超离心法相比,直流过使电动汽车生产大幅增加.
  • 鉴定证实了形态,大小,蛋白质标记物 (ALIX,TSG101,CD63,CD81) 和成功加载纳米粒子在EVs.
  • 与自由纳米粒子相比,装载纳米粒子的EV显示出增强和更均的细胞吸收,这表明传递效果更好.

结论:

  • 直接流过是一种可行和可扩展的方法,用于从癌细胞中产生纳米粒子载荷的EV.
  • 纳米粒子载荷EV的增强细胞吸收表明它们在药物输送应用中具有更深层次的组织透和改善治疗疗效的潜力.