基于交联素和低分子量氨酸的复合水凝用于组织工程
Maria Drozdova1, Marina Vodyakova1, Tatiana Tolstova1
1Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya Str., 117997 Moscow, Russia.
Polymers
|May 27, 2023
概括
这项研究开发了用于组织工程的奇托桑/氨酸水凝. 批量修改增强了细胞生长和增殖,显示出作为生物降解基质的承诺.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 聚合物化学 聚合物化学
背景情况:
- 素 (Ch) 和氨酸 (HA) 是生物相容的聚合物,在组织工程中具有潜力.
- 开发有效的生物降解矩阵对于再生医学至关重要.
- 控制水凝结构和特性影响细胞行为.
研究的目的:
- 使用两种不同的制备方法制造巨孔奇托桑/氨酸 (Ch/HA) 水凝.
- 描述这些Ch/HA水凝的结构和性能概况.
- 评估 Ch/HA 水凝作为组织工程应用中的可生物降解矩阵的体外有效性.
主要方法:
- 通过对Ch与基尼或甲进行共价交联来制造宏的Ch/HA水凝.
- 两个修改方法:散装 (方法1) 和表面 (方法2) 纳入低分子量HA (5和30kDa).
- 使用共聚焦激光扫描显微镜 (CLSM) 进行表征,并用小鼠纤维细胞 (L929) 评估胀,降解和细胞活力 (MTT测定).
主要成果:
- 成功地制造出高度多孔,相互连接的结构 (50-450微米孔).
- 与单独的Ch矩阵相比,低分子量HA的捕获显著增强了细胞生长.
- 通过散装修改 (第1方法) 制备的Ch/HA水凝表现出比表面修改 (第2方法) 更好的细胞粘附,生长和增殖.
结论:
- 巨孔的Ch/HA水凝可以使用散装或表面修饰技术制备.
- 批量修改低分子量HA的Ch/HA水凝,促进细胞反应的增强.
- 这些Ch/HA水凝显示出作为组织工程应用的可生物降解矩阵的巨大潜力.
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