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电化疗对瘤细胞免疫学上重要的修改的影响
Ursa Kesar1,2, Bostjan Markelc1, Tanja Jesenko1,2
1Department of Experimental Oncology, Institute of Oncology Ljubljana, 1000 Ljubljana, Slovenia.
Vaccines
|May 27, 2023
概括
使用白素,CDDP或OXA的电化学疗法 (ECT) 诱导免疫细胞死亡 (ICD) 标志物. 特定的ICD签名和免疫标记物变化因癌细胞系和药物度而异.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
背景情况:
- 电化学疗法 (ECT) 将化疗与电脉冲相结合,以增强药物输送.
- 基于白素 (BLM) 的ECT可以诱导免疫细胞死亡 (ICD),但其在癌症类型和其他药物中的普遍性尚不清楚.
研究的目的:
- 研究ECT诱导的ICD相关损伤相关分子模式 (DAMPs) 和免疫标记物的变化.
- 根据癌细胞系和化疗剂来评估这些变化的特异性.
主要方法:
- 在体外评估B16-F10,4T1和CT26小鼠瘤细胞系.
- 在ECT后对DAMP (Calreticulin,ATP,HMGB1) 和免疫标记物 (MHCI,MHC II,PD-L1,CD40) 的分析.
- 在治疗后长达48小时的时间过程分析.
主要成果:
- 使用西斯 (CDDP),氧沙 (OXA) 或BLM诱导的ICD相关的DAMPs的ECT.
- 免疫标记的DAMP特征和表达是特定于细胞系和药物度的.
- 在细胞系和度依赖的方式中,ECT调节了MHCI,MHC II,PD-L1和CD40的表达.
结论:
- 带有临床相关化疗剂 (CDDP,OXA,BLM) 的ECT可以诱导ICD.
- 对ECT的免疫反应取决于情境,因癌症类型和药物度而异.
- 在癌症治疗中,ECT代表了一种诱导免疫细胞死亡的有希望的策略.
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