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使用单细胞滴滴数字PCR进行重组病毒量化:一种用于传染性titer量化的方法

Ksenija Korotkaja1, Anna Zajakina1

  • 1Cancer Gene Therapy Group, Latvian Biomedical Research and Study Centre, Ratsupites Str. 1, k.1, LV-1067 Riga, Latvia.

Viruses
|May 27, 2023
PubMed
概括

这项研究引入了一种新的滴滴数字PCR (ddPCR) 方法,用于量化Semliki森林病毒 (SFV) 载体. 该技术准确地测量病毒基因组标位和感染单位,为研究和临床使用提供了稳定和可复制的替代方案.

关键词:
塞米利基森林病毒阿尔法病毒的病毒.ddPCRR 在线播放传染性标位的传染性标位.复制缺陷的病毒颗粒.一个单细胞ddPCR.

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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