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2016年至2017年间在越南活鸟市场分离的高致病性H5型流感病毒

Lizheng Guan¹, Gongxun Zhong¹, Shufang Fan¹

¹Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53711, USA.

|May 27, 2023

概括

在越南检测到高致病性禽流感H5N1和H5N6病毒. 类2.3.2.1c病毒在小鼠中引起致命感染,与其他类不同.

科学领域:

兽医病毒学兽医病毒学
分子流行病学分子流行病学
传染性疾病传染性疾病

背景情况:

常规监测活禽市场对于监测禽流感病毒 (AIV) 演变至关重要.
高致病性禽流感 (HPAI) 病毒,特别是H5N1和H5N6,对家禽健康和潜在的公共健康构成重大威胁.
了解病毒遗传多样性和重组是预测和控制疫情的关键.

研究的目的:

描述2016年至2017年间从越南北部分离出来的HPAI H5N1和H5N6病毒的遗传多样性和遗传关系.
为了调查潜在的重组事件与低致病性禽流感病毒.
在小鼠模型中评估不同病毒类型的致病性.

主要方法:

从家禽监测中对HPAI病毒进行隔离和全基因组测序.
植物遗传学分析以确定病毒类和进化关系.
深度测序用于识别小病毒亚群和遗传变异.
感染小鼠模型,以评估不同病毒类的病原性.

主要成果:

隔离了27种HPAI H5N1和H5N6病毒,这些病毒属于2.3.2.1c,2.3.4.4f和2.3.4.4g类.
遗传学分析表明HPAI病毒和低致病性禽流感病毒之间发生了重组事件.
深度测序揭示了微小的病毒亚群,对病原性和抗病毒药物敏感性有潜在的影响.
在体内研究表明,类2.3.2.1c病毒在小鼠中引起致命感染,而类2.3.4.4f和2.3.4.4g导致非致命感染.

结论:

越南家禽市场中各种HPAI H5N1和H5N6菌株的流通凸显了正在进行的病毒演变和重新分类.
在哺乳动物模型中,例如2.3.2.1c等特定类型表现出明显的致病潜力,因此需要有针对性的监测.
识别小病毒变异表明需要先进的测序技术,以充分了解病毒风险.

关键词:

H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H5 H3 H5 H5 H5 H5 H3 H5 H5 H3 H4 H5 H5 H5 H5 H3 H5 H3 H4 H5 H6 H7 H8 H8 H8 H8 H8 H8 H9 在线阅读越南越南越南越南深度测序是一种深度测序.流感病毒是流感的病毒.鼠标鼠标鼠标鼠标监控监督监督监督监督监督监督监督监督监督监督监督监督监督监督监督监督监督

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