参与miRNA生物发生的基因在SARS-CoV-2感染中没有下调
Nathalie Garnier1,2, Famara Sane1, Layal Massara3
1Laboratoire de Virologie ULR3610, University Lille and CHU Lille, F-59000 Lille, France.
Viruses
|May 27, 2023
概括
严重的COVID-19患者没有显示关键miRNA生物发生基因信使RNAs (mRNAs) 的水平降低. 在大多数测试的细胞或患者样本中,SARS-CoV-2感染并没有显著改变这些mRNA水平.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 遗传学 是一个遗传学.
背景情况:
- 微RNA (miRNA) 是小型非编码RNA,对基因调节至关重要,并与病毒感染有关.
- 病毒感染可以通过抑制参与miRNA生物发生的基因来破坏miRNA路径.
- 之前的观察表明,在严重的COVID-19中,miRNA水平降低,暗示它们作为生物标志物的潜力.
研究的目的:
- 研究SARS-CoV-2感染对关键miRNA生物发生基因的信使RNA (mRNA) 表达水平的影响.
- 为了确定SARS-CoV-2是否影响诸如AGO2,DICER1,DGCR8,DROSHA和XPO5.5等基因.
主要方法:
- 使用定量逆转录聚合酶链反应 (RT-qPCR) 来测量mRNA水平.
- 样本包括来自COVID-19患者 (严重和不严重) 和对照组的鼻口腔抽样.
- 在体外实验中,涉及到NHBE,Calu-3和Vero E6细胞的SARS-CoV-2感染.
主要成果:
- 在严重的COVID-19患者,非严重的病例和对照组之间,没有发现AGO2,DICER1,DGCR8,DROSHA和XPO5mRNA水平的显著差异.
- SARS-CoV-2 感染并没有显著改变 NHBE 和 Calu-3 细胞中这些基因的表达.
- 在感染后24小时内,Vero E6细胞中观察到AGO2,DICER1,DGCR8和XPO5mRNA的轻微上调.
结论:
- 该研究没有发现患者样本或大多数细胞系中SARS-CoV-2感染期间关键miRNA生物发生基因的mRNA下调的证据.
- 这些发现表明,SARS-CoV-2不会在这些特定基因的mRNA水平上广泛抑制miRNA生物发生.
- 在Vero E6细胞中观察到的轻微上调值得进一步调查,但不支持SARS-CoV-2对miRNA生物发生基因的一般抑制机制.
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