使用纳米孔直接RNA测序分析完整的mRNA多A尾长的协议

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Updated: Jul 29, 2025

Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques
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Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques

Published on: January 12, 2024

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Koichi Ogami1, Yuka Oishi2, Shin-Ichi Hoshino2

  • 1Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603 Japan; Division of Molecular Oncology, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.

STAR protocols
|May 27, 2023
PubMed
概括

这项研究引入了一种纳米孔测序方法,以准确测量mRNA多类的尾巴长度. 这种技术可以对基因表达,RNA拼接和修改进行全面分析.

关键词:
细胞生物学 细胞生物学分子生物学分子生物学

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