在Bacillus中删除Rap-酸酶以控制定数感应及其对表面因子生产的影响
Chantal Treinen1,2, Lennart Biermann1, Maliheh Vahidinasab1
1Institute of Food Science and Biotechnology, Department of Bioprocess Engineering (150k), University of Hohenheim, Fruwirthstr. 12, 70599, Stuttgart, Germany.
AMB Express
|May 27, 2023
概括
在 Bacillus subtilis 中删除特定的基因 (rapC,rapF,rapH) 会增强表面素的产生,表面素是一种有价值的脂. 这项研究表明,通过定数感应操纵来控制生物过程的潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 合成生物学 合成生物学
背景情况:
- 细菌的定数感应调节基因表达,影响生物过程.
- 该PsrfA促进体是生产脂表面素的关键.
- 拉普酸酶 (RapC,RapF,RapH) 影响PsrfA活动.
研究的目的:
- 调查删除rapC,rapF和rapH基因对Bacillus subtilis.surfactin产生的影响.
- 为了确定操纵定数感应组件是否可以提高脂产量.
- 评估这些遗传修饰对生物过程控制的潜力.
主要方法:
- 在一个 *sfp*+ *B. subtilis* 168衍生物中删除了rapC,rapF和rapH的基因.
- 在延长种植时间内对表面素生产的定量评估.
- 测量产品每种生物质的产量 (YP/X) 和特定表面素生产率 (qsurfactin).
- 评估ComX活动和PsrfA促进器活动 (使用PsrfA-lacZ).
主要成果:
- 与基准菌株相比,删除突变 (ΔrapC, ΔrapF) 在24小时培养后显示表面素标位显著增加 (分别是2.7倍和2.5倍).
- 在ΔrapC和ΔrapF菌株中观察到,每种生物质的产量提高 (YP/X) 和特定表面素生产率 (q表面素).
- 虽然 ΔrapH 菌株表现出高促进子活性,但其表面素定位的增加不那么明显.
- 基因删除在很大程度上没有影响ComX活性.
结论:
- 删除特定的Rap-酸酶基因 (rapC,rapF) 有效地提高了Bacillus subtilis*中的表面素的产生.
- 这项研究验证了这样一个假设:操纵定数感应元件可以提高脂产量.
- 这些发现支持对量子感应系统调制的应用,以优化生物过程,特别是在脂生产中.
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