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在湿干循环下使用2'-3'循环核酸进行非酶型模板定向的原料扩展

Shikha Dagar¹, Susovan Sarkar¹, Sudha Rajamani²

¹Department of Biology, Indian Institute of Science Education and Research, Pune, 411008, India.

Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life

|May 27, 2023

概括

这项研究表明,在恶劣的条件下,使用与前生物相关的循环核酸进行非酶性RNA原料扩展. 这一发现支持RNA世界假设,因为它显示了早期RNA复制的可信机制.

科学领域:

生命的起源研究生命的起源研究
益生菌化学益生菌化学
分子进化分子进化

背景情况:

RNA世界假设提出了早期的RNA分子作为遗传物质和催化剂的作用.
以前的模板导向RNA合成通常需要激活的核酸或导致基底部位.
非酶性RNA复制对于理解生命起源至关重要.

研究的目的:

通过使用前生物相关的循环核酸来研究非酶型模板导向的原料扩展.
在模拟的早期地球条件下探索RNA复制的可行性.
确定有利于高效RNA单体聚合的条件.

主要方法:

在高温 (90°C) 和性pH (8) 的情况下利用了脱水-再水 (DH-RH) 循环.
采用2'-3'循环核酸单酸盐 (cNMPs) 作为原料扩展的单体.
测试了正规基终结 (OH-primer) 和氨基终结 (NH2-primer) 原料.
研究了脂质对反应效率的影响.

主要成果:

通过使用2'-3'cNMPs,但不是3'-5'cNMPs,成功实现了模板定向的原料扩展.
在OH-和NH2-原始物中观察到多达两种核酸添加.
purin 和 pyrimidine 2'-3' cNMP 都支持了原料扩展,cAMP 产生了更高的产品.
脂质在循环CMP反应中显著增强了扩展产品产量.

结论:

这项研究提供了使用内在激活的循环核酸进行非酶性RNA原料扩展的概念证明.
这些发现支持早期地球环境中RNA复制的合理性.
循环核酸和脂质是预微生物RNA合成的关键组成部分.

关键词:

循环核酸是循环核酸的一种.脱水 - 重水循环的脱水 - 重水循环益生菌前生物化学线程分离的分离方式模板导向的初始化扩展程序扩展

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