在植物动力学中,运输偏差和Arabidopsis中移动siRNAs的内生功能
Emanuel A Devers1, Christopher A Brosnan1, Alexis Sarazin1
1Department of Biology, Swiss Federal Institute of Technology (ETH), Universitätsstrasse 2, 8092, Zürich, Switzerland.
The Plant journal : for cell and molecular biology
|May 27, 2023
概括
植物中的RNA干扰 (RNAi) 运动由等离子体 (PDs) 和ARGONAUTE (AGO) 蛋白调节. 这项研究揭示了小干扰RNAs (siRNAs) 如何系统移动以及它们在基因调节中的潜在作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因调节 基因调节
- RNA干扰机制的RNA干扰机制
背景情况:
- RNA干扰 (RNAi) 利用小干扰RNAs (siRNAs) 和ARGONAUTE (AGO) 蛋白质进行基因沉默.
- 植物中的系统性RNAi运动通过等离子体 (PDs) 发生,但机制和siRNA选择性仍然不清楚.
- 了解移动RNAi对于其应用和破译内源功能至关重要.
研究的目的:
- 为了研究等离子体 (PDs) 在系统性RNAi传输中的作用.
- 阐明影响血管运动期间siRNA大小选择性的因素.
- 探索内源性siRNAs (内-siRNAs) 的运动和调节潜力.
主要方法:
- 在Arabidopsis的伴侣细胞 (CCs) 中局部PD封闭,以阻止转基因沉默.
- 对siRNA长度选择性的分析与不同组织中AGO蛋白质存在的关系.
- 使用压力条件,诱导内-siRNA转位对体流动的诱导.
- 转录组分析以确定移动性内分泌siRNAs的目标.
主要成果:
- 包括源叶中的PDs CCs 废除了水槽叶中的系统性转基因沉默.
- 特定的AGO蛋白质会影响血管运输过程中siRNA大小的选择.
- 压力条件使特定的内源-siRNAs能够转移到特定的内源-siRNAs,而不是从芽到根的流动.
- 来自反向重复 (IR) 位点的移动内核siRNAs调节了数百个转录.
结论:
- 在伴侣细胞中的等离子体关口对于系统性RNAi至关重要.
- 在运动过程中,AGO蛋白质在确定siRNA大小选择性方面发挥着关键作用.
- 内分RNA的移动性可以被操纵,即使与正常流动相抵触,也会影响基因调节.
- 这项研究阐明了移动RNAi机制,并为未来的研究提供了基础.
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