甲基的微生物工程激活AM1以增强CO2转化为形式的转化
Uyen Thu Phan1, Byoung Wook Jeon1, Yong Hwan Kim1
1School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 44919, the Republic of Korea.
Enzyme and microbial technology
|May 27, 2023
概括
这项研究对Methylorubrum extorquens AM1进行了改进,以改善二氧化碳 (CO2) 的转化. 优化的终端子和5'-未翻译区域 (5'-UTR) 显著提高了甲酸脱酶表达和甲酸生成.
科学领域:
- 合成生物学 合成生物学
- 生物催化剂是一种生物催化剂.
- 代谢工程是代谢工程.
背景情况:
- 甲基虫 AM1 是一种多功能C1原料消费者,用于生物材料生产.
- 精确控制重组酶的表达对于工程M. extorquens AM1.1.的精确控制至关重要.
- 开发对M. extorquens AM1有效的工具对于其生物技术应用至关重要.
研究的目的:
- 为了增强M. extorquens AM1.1.中的甲酸盐脱酶1 (MeFDH1) 的表达.
- 使用全细胞生物催化剂改善二氧化碳 (CO2) 转化活动.
- 研究终端子和5 - 未翻译区域 (5 -UTR) 设计对MeFDH1表达和CO2转换的影响.
主要方法:
- 设计并测试了MeFDH1亚单元mRNA和蛋白质表达的高效终结器 (rrnB与T7).
- 根据蛋白质组学和UTR设计工具识别和使用同类的5-UTR (例如来自fae).
- 评估了使用工程全细胞生物催化剂将二氧化碳电化学转化为形成的过程.
主要成果:
- rrnB终结器将MeFDH1mRNA水平提高了8.2倍 (α) 和11倍 (β),酶产量增加了1.6倍.
- 与对照组相比,甲激活酶 (fae) 5 -UTR导致MeFDH1表达增加了2.5倍.
- 工程菌株显示了增强的二氧化碳转化,fae 5 -UTR菌株实现了5.0mM/h的形式生产率 (2.3倍以上).
结论:
- 有效的终结器和5-UTR设计显著增强了M. extorquens AM1.1中的重组酶表达.
- 在M. extorquens AM1中优化了MeFDH1表达,使得二氧化碳转化为可生物利用的格式得到了改进.
- 这项研究为生物技术应用在甲基型细菌中开发强大的复合表达系统提供了宝贵的见解.
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