拉普1协调细胞-细胞粘附和细胞骨重组,以在体内推动集体细胞迁移
Katheryn E Rothenberg1, Yujun Chen2, Jocelyn A McDonald2
1Institute of Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada; Translational Biology and Engineering Program, Ted Rogers Centre for Heart Research, University of Toronto, Toronto, ON M5G 1M1, Canada.
Current biology : CB
|May 27, 2023
概括
小型GTPase Rap1通过组织细胞粘附和细胞骨动力学来协调胚胎伤口修复. 拉普1通过粘附结重塑和actomyosin电缆组装确保了快速的集体细胞迁移.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 生物物理学的生物物理.
背景情况:
- 集体细胞迁移对于组织发育,修复和疾病至关重要.
- 表皮中的凝聚性细胞运动取决于附着结和actomyosin细胞骨架.
- 在体内集体迁移过程中细胞粘附和细胞骨重塑的协调机制尚不清楚.
研究的目的:
- 在体内研究协调细胞-细胞粘附和细胞骨重塑在集体细胞迁移过程中的机制.
- 阐明小GTPase Rap1在胚胎上皮层伤口愈合中的作用.
- 确定关键的信号通路和参与快速伤口修复的分子参与者.
主要方法:
- 利用Drosophila胚胎研究表皮伤口愈合.
- 采用基因操纵来表达Rap1因子的突变形式.
- 通过显微镜和生化分析研究了蛋白质的定位和激活.
主要成果:
- 拉普1对于快速的伤口修复是必要和充分的,促进肌两极分化和E-cadherin的积累.
- 通过Canoe/Afadin传递Rap1信号,用于粘附接口改造.
- 拉普1通过Ephexin激活RhoA/Rho1,这对于actomyosin电缆组装和伤口修复至关重要.
结论:
- 在胚胎伤口愈合过程中,Rap1充当分子重组的中心协调员.
- 通过Ephexin-Rho1通道,Rap1协调了通过Ephexin-Rho1通道进行的actomyosin电缆组装.
- 拉普1通过Canoe促进了E-cadherin的再分配,使得在体内迅速的集体细胞迁移成为可能.
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