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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Comparing Copy Number Variations and SNPs

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...

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Updated: Jul 29, 2025

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

C4S DB:为ChIP-Seq数据库进行全面的收集和比较

Hayato Anzawa¹, Kengo Kinoshita²

¹Department of Integrative Genomics, Tohoku Medical Megabank Organization, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8573, Japan; Department of System Bioinformatics, Graduate School of Information Sciences, Tohoku University, 6-3-09, Aramaki-Aza-Aoba, Aoba-ku, Sendai 980-8579, Japan.

Journal of molecular biology

|May 27, 2023

概括

此摘要是机器生成的。

对ChIP-seq数据库的综合收集和比较 (C4S DB) 集成了超过16000个ChIP-seq实验. 它使研究人员能够探索组合的DNA结合元素配置文件和监管关系.

关键词:

它们是DNA结合蛋白质.基质子的修改基质子的修改质量控制质量控制质量控制转录因子是一种转录因子.转录规则转录规则转录规则

更多相关视频

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

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Comparative Lesions Analysis Through a Targeted Sequencing Approach

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

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Last Updated: Jul 29, 2025

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

理解复杂的生物系统需要整合多个结合特征,包括转录因子和基因组修饰.
现有的染色体免疫沉和其后的测序 (ChIP-seq) 数据库通常集中在单个实验上,阻碍了DNA结合元素协调调节的分析.

研究的目的:

开发一个全面的数据库 (C4S DB) 用于分析使用质量评估的公开ChIP-seq数据的组合DNA结合元件配置文件.
为发现ChIP-seq实验之间的关系提供交互式网络接口,促进对基因调节的研究.

主要方法:

汇总和质量评估了超过16000个人类ChIP-seq实验.
开发了一个"基因浏览器"来可视化特定基因周围的结合元素分布.
实施了一个"全球相似性"功能,使用层次聚类热图来分析ChIP-seq实验之间的全基因组关系.

主要成果:

C4S DB提供了工具来识别基因特异性和全基因组共定位或监管元素的相互排斥的定位.
交互式网络接口可以有效地搜索和聚合大规模的ChIP-seq数据.
该数据库有助于评估监管要素的相互作用.

结论:

C4S DB提供了对DNA结合元素的组合性和它们的调节作用的宝贵见解.
该资源增强了研究人员通过集成的ChIP-seq数据分析来阐明复杂的基因调节机制的能力.