在 Shewanella oneidensis MR-1 中使用 Arc 依存的转录促进体进行基因表达的电遗传控制
Keisuke Tomita1, Atsumi Hirose1, Yugo Tanaka1
1School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Journal of bioscience and bioengineering
|May 27, 2023
概括
研究人员在电化学活性细菌 (EAB) 中确定了电极潜在响应促进体. 这些由弧形系统调节的促进体使基因表达的电控制成为可能,为生物电化学系统 (BES) 中的电遗传学铺平了道路.
科学领域:
- 微生物学 微生物学
- 生物工程是生物工程.
- 合成生物学 合成生物学
背景情况:
- 电化学活性细菌 (EAB) 与电极相互作用,对生物电化学系统 (BES) 至关重要.
- 控制EAB代谢活动是推动BES应用的关键.
- 在Shewanella oneidensis MR-1中,弧形系统根据电极潜力调节基因表达,这表明电气控制的潜力.
研究的目的:
- 在EAB中识别和表征电极电位响应,弧度依赖的转录促进体.
- 通过使用这些促进器来电气控制基因表达来探索电遗传学的可行性.
主要方法:
- 在Shewanella oneidensis MR-1和Escherichia coli基因组中探索了Arc-依赖的促进体.
- 使用的LacZ报告员测定与电极相关的MR-1细胞接触到不同的电极电位 (+0.7V和-0.4V).
- 开发了一种微观系统,用于现场监测电极关联细胞中的促进子活性.
主要成果:
- 确定了E. coli feo (Pfeo) 和MR-1 nqrA2 (Pnqr2) 基因上游的促进体,作为电极电位响应.
- 在S. oneidensis MR-1中,Pfeo活动在+0.7V时增加,而Pnqr2活动在-0.4V时增加.
- 在MR-1细胞中,Pnqr2在0.4V时显示持续诱导,通过实地监测证实了这一点.
结论:
- 电极电位响应促进剂使EAB中的基因表达能够有效地通过电气调节.
- 这些促进子为开发电遗传学提供了基础的分子工具.
- 这些发现推动了EAB在生物电化学系统中的潜在应用.
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