长非编码RNAZFAS1通过miR200b/ZEB1轴促进结直肠癌的进展
Islam T Salman1, Ahmed I Abulsoud2, Dina M Abo-Elmatty1
1Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
Pathology, research and practice
|May 28, 2023
概括
大肠直肠癌 (CRC) 的进展涉及长非编码RNA ZFAS1,microRNA-200b和ZEB1蛋白. 它们的相关性表明,它们有可能作为诊断生物标志物和CRC的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 大肠直肠癌 (CRC) 是一种普遍存在的全球性恶性瘤.
- 微RNA (miRNA) 和长非编码RNA (lncRNA) 在CRC发育中起着重要的作用.
- 在CRC中,lncRNA ZFAS1,miR-200b和ZEB1蛋白之间的相互作用需要进一步阐明.
研究的目的:
- 在结肠直肠癌患者中调查lncRNA ZFAS1,miR-200b和ZEB1蛋白的血清水平之间的相关性.
- 评估这些分子作为CRC的诊断生物标记物的潜力.
- 探索ZFAS1/miR-200b/ZEB1轴在CRC进展中的作用.
主要方法:
- 定量实时聚合酶连锁反应 (qRT-PCR) 用于血清lncRNA ZFAS1和microRNA-200b表达分析.
- 与酶相关的免疫吸收试验 (ELISA) 用于血清ZEB1蛋白质的量化.
- 60名CRC患者和28名健康对照人群之间的比较分析.
主要成果:
- 与对照组相比,血清lncRNA ZFAS1和ZEB1蛋白在CRC患者中显著上调.
- 在CRC患者中,血清miR-200b显著下调.
- 在CRC患者中观察到ZFAS1表达,miR-200b水平和ZEB1蛋白之间存在显著的线性相关性.
结论:
- lncRNA ZFAS1作为结直肠癌进展的关键因素,可能通过海绵化miR-200b.
- 该ZFAS1/miR-200b/ZEB1通路呈现了一个有前途的新型诊断生物标志物签名的人类CRC.
- 向ZFAS1可能为结直肠癌提供一种潜在的治疗策略.
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