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Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
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在牛IVEP中使用微流体精子分类芯片是否会影响胚胎细胞的发育?

Hasan Alkan1, Fatma Satilmis1, Mehmet Akif Demirel1

  • 1Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey.

Reproduction in domestic animals = Zuchthygiene
|May 29, 2023
PubMed
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微流体精子分类芯片改善了牛胚胎发育和体外胚胎生产 (IVEP) 的质量. 与常规的精子治疗相比,这种方法增加了胚胎细胞的发育率并减少了亡.

科学领域:

  • 生殖生物学 生殖生物学
  • 动物科学动物科学
  • 生物技术是生物技术.

背景情况:

  • 在牛体外胚胎生产 (IVEP) 对于遗传改进至关重要.
  • 精子质量显著影响IVEP成功率.
  • 目前的精子制备方法可能无法优化精子选择用于受精.

研究的目的:

  • 评估微流体精子分类芯片 (MFSC) 在牛IVEP期间改善胚胎发育和质量的有效性.
  • 为了比较使用MFSC准备精子与常规精子治疗的结果.

主要方法:

  • 荷尔斯坦牛卵细胞在体外成熟,并随机分配到两个组.
  • 实验小组使用了经过微流体精子分类芯片 (MFSC) 处理的精子.
  • 对照组使用了经过商业公司标准协议准备的精子.

主要成果:

  • 在MFSC组显示显著更高的裂变率 (85.71%对比76.33%) 和胚胎细胞发育率 (44.15%对比32.54%).
  • 来自MFSC组的胚胎表现出内部细胞质量 (ICM),体 (TE) 和总细胞 (TC) 的细胞数量增加.
  • 从MFSC治疗的精子 (3.06%对比7.72%) 衍生出的胚芽细胞中,亡率明显较低.
关键词:
在IVEPEP中使用IVEP.灭症 (apoptosis) 是一种死亡的过程.裂变率是指裂变的速度是多少.达到囊胚细胞的速度.精子治疗 精子治疗

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结论:

  • 微流体精子分类芯片技术提高了牛IVEP的胚胎发育,质量和胚芽细胞形成率.
  • MFSC可降低牛胚胎发育中的细胞亡,这表明雌性体质量有所改善.
  • 这项技术为牛IVEP精子制备提供了一个有希望的替代方案.