对在辛德邦 (Sindh) 巴基斯坦流传的Vibrio cholerae O1 El Tor变种的评估
Amjad Ali Mughal1, Yasmeen Faiz Kazi1, Habib Bokhari2
1Institute of Microbiology, Shah Abdul Latif University Khairpur, Khairpur.
概括
在巴基斯坦的Khairpur农村地区发现了基因多样化的Vibrio cholerae O1 El Tor变种菌株. 遗传学分析显示,这些土著菌株与其他循环的Vibrio cholerae有共同的祖先.
科学领域:
- 微生物学 微生物学
- 遗传学 遗传学 是一个
- 流行病学 流行病学
背景情况:
- 在全球范围内,霍乱仍然是一个重大的公共卫生问题.
- 了解Vibrio cholerae的遗传多样性对于有效的疾病控制至关重要.
研究的目的:
- 在巴基斯坦Khairpur调查Vibrio cholerae菌株的遗传多样性.
- 为了确定本土Vibrio cholerae菌株的遗传关系.
主要方法:
- 一项涉及2014年4月至2016年5月收集的360个便/直肠样本的横截面研究.
- 使用微生物学,生物化学,血清学和PCR方法 (ompW基因) 进行鉴定.
- 全基因组测序和生物信息学分析 (MUMmer 3.2.3) 用于菌株比较和家族遗传树构造 (邻居连接方法).
主要成果:
- 76个 (21.11%) 样本对Vibrio cholerae菌株呈阳性,被确定为血清组Inaba,O1,生物型El Tor.
- 全基因组测序揭示了与参考序列相比,测试菌株中的独特序列.
- 遗传学分析表明,所有分离的菌株都来自与参考菌株相同的祖先,局部分离物之间有一些差异.
结论:
- 霍乱病毒O1埃尔托尔变种菌株存在于巴基斯坦Khairpur.
- 这项研究强调了基因多样化和本地适应的Vibrio cholerae菌株的存在.
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