相关实验视频
Updated: Jul 28, 2025

08:26
Monitoring Stub1-Mediated Pexophagy
Published on: May 12, 2023
1.6K
监测Stub1介导的食
1Institute of Biological Chemistry, Academia Sinica.
Journal of visualized experiments : JoVE
|May 29, 2023
概括
哺乳动物细胞使用Stub1介导的食法去除过氧体. 这个过程可以使用产生反应性氧物种 (ROS) 的染料来触发和监控,从而控制过氧体数量和质量.
科学领域:
- 细胞生物学 细胞生物学
- 自学研究 自学研究
- 组织质量控制机构质量控制机构
背景情况:
- 哺乳动物细胞具有过氧体循环的机制,包括Stub1介导的食.
- 这一途径允许细胞调节过氧体数和完整性.
- 热冲击蛋白70和E3结合酶Stub1是启动食在转移到过氧体时的关键组成部分.
研究的目的:
- 详细介绍了使用染料辅助反应性氧物种 (ROS) 生成的Stub1介导食的启动和监测方法.
- 为利用光蛋白和合成光体来触发哺乳动物细胞培养中的食性提供协议.
- 为了证明在细胞群内或单个细胞内针对全球性向过氧体的能力.
主要方法:
- 使用Stub1 (压力诱导蛋白1) 作为E3无素化酶来调节食.
- 使用染料辅助生成反应性氧物种 (ROS) 来激活食路径.
- 实施活细胞显微镜技术,观察Stub1介导的食动态.
主要成果:
- 斯图布1酶活性促进了在向的过氧体上积聚乌比奎丁和与自相关的因素.
- 过氧体内ROS水平升高会激活Stub1介导的食.
- 使用光染料的协议使细胞培养中可控启动和监测pexophagy.
结论:
- 通过Stub1介导的食是一种可行的途径,用于控制哺乳动物细胞中的过氧体同质性.
- 染料辅助的ROS生成提供了一个多功能工具,可以在细胞和亚细胞水平上启动和研究食.
- 活细胞成像与基于染料的ROS诱导相结合,为研究器官循环机制提供了强大的方法.
相关概念视频
Export of Misfolded Proteins out of the ER
3.7K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.7K
Protein Import into the Peroxisomes
3.6K
Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
3.6K
ER Retrieval Pathway
3.9K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
3.9K
Delivery Pathways to the Lysosome
6.6K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.6K
Autophagy
4.4K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.4K

