通过代谢工程Corynebacterium glutamicum进行基于formamide的氨基酸生产
Lynn S Schwardmann1, Tong Wu2, Aron K Dransfeld1
1Genetics of Prokaryotes, Faculty of Biology and CeBiTec, Bielefeld University, Universitätsstr. 25, 33615, Bielefeld, Germany.
Applied microbiology and biotechnology
|May 29, 2023
概括
科里尼细菌谷氨酸被设计为利用形式胺作为源,使得生产有价值的化合物成为可能. 这一进步支持非无菌生物生产和交叉养策略.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 代谢工程是代谢工程.
背景情况:
- 微生物利用形式胺作为源是有限的.
- 甲胺和甲胺酶可以作为非无菌生物生产的保护系统.
- 谷氨酸菌 (Corynebacterium glutamicum) 是一种成熟的工业微生物,用于氨基酸生产.
研究的目的:
- 改造Corynebacterium glutamicum以利用形式胺作为其唯一的源.
- 建立基于形式胺的各种化合物的生产.
- 探索甲基胺/甲基胺酶系统在微生物生物生产中的潜力.
主要方法:
- 用Helicobacter pylori formamidase对Corynebacterium glutamicum进行基因工程.
- 使用甲胺作为唯一源的工程菌株的培养.
- 稳定同位素标记以追踪的结合.
- 共同培养实验以证明交叉养.
- 过度表达甲酸脱酶以增强甲胺利用率.
主要成果:
- 工程C. glutamicum成功地使用形式胺作为唯一的源生长.
- 实现了基于formamide的L-glutamate,L-lysine,N-methylphenylalanine和二林酸的生产.
- 稳定同位素标记证实了从生物质和L-lysine中纳入的formamide中的.
- 从formamide代谢中泄漏的氨酸支持了formamidase缺乏菌株在共同培养中的生长.
- 甲酸脱酶的过度表达提高了甲胺利用效率.
结论:
- 甲胺/甲胺酶系统使得C. glutamicum能够使用甲胺作为唯一的源.
- 这种工程系统有助于高效生产各种化合物.
- 该系统提供了非无菌生物生产和代谢工程策略的潜力,涉及交叉养.
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