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Updated: Jul 28, 2025

Author Spotlight: Investigating Physiological Functions of Vitamin A Transporters Using HPLC-Based Vitamin A Profiling

Author Spotlight: Investigating Physiological Functions of Vitamin A Transporters Using HPLC-Based Vitamin A Profiling

Published on: December 27, 2024

通过轨道轨道质谱仪进行雷替尼埃斯特分析.

Jeroen W A Jansen¹, Maya W Haaker¹, Esther A Zaal¹

¹Division of Cell Biology, Metabolism & Cancer, Faculty of Veterinary Medicine, Department of Biomolecular Health Sciences, Utrecht University, Utrecht, The Netherlands.

Methods in molecular biology (Clifton, N.J.)

|May 29, 2023

概括

我们开发了一种使用高分辨率质谱的新方法,用于敏感检测和定量网乙. 这种技术可以准确地描述生物样本中的这些光敏感分子.

关键词:

大气压力化学电离化学电离高性能液体色谱学高性能液体色谱学质谱测量质量谱测量轨道上的陷网红醇是什么网红醇网烯 (Retinil esters) 是一种类型的烯.维生素A 维生素A是一种维生素.

更多相关视频

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein

Published on: January 28, 2013

Author Spotlight: Unraveling Vitamin A Transport Mechanisms — Linking Liver Receptors to Vision Health Through RBPR2 and RBP4 Interactions

Author Spotlight: Unraveling Vitamin A Transport Mechanisms — Linking Liver Receptors to Vision Health Through RBPR2 and RBP4 Interactions

Published on: October 4, 2024

相关实验视频

Last Updated: Jul 28, 2025

Author Spotlight: Investigating Physiological Functions of Vitamin A Transporters Using HPLC-Based Vitamin A Profiling

Author Spotlight: Investigating Physiological Functions of Vitamin A Transporters Using HPLC-Based Vitamin A Profiling

Published on: December 27, 2024

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein

Published on: January 28, 2013

Author Spotlight: Unraveling Vitamin A Transport Mechanisms — Linking Liver Receptors to Vision Health Through RBPR2 and RBP4 Interactions

Author Spotlight: Unraveling Vitamin A Transport Mechanisms — Linking Liver Receptors to Vision Health Through RBPR2 and RBP4 Interactions

Published on: October 4, 2024

科学领域:

生物化学生物化学
分析化学分析化学
分子生物学分子生物学

背景情况:

视网体是必不可少的生物分子,但它们的检测通常依赖于紫外线吸收.
现有的方法可能缺乏灵敏度或特异性,无法进行详细的网乙烯分析.
在各种生物环境中,了解乙烯 Ester 概况至关重要.

研究的目的:

建立一种新的,高度敏感的方法来识别和量化网乙物种.
在复杂的生物矩阵中表征网乙.
推进用于视网类药物研究的分析技术.

主要方法:

使用Bligh和Dyer方法提取网乙.
通过高性能液体染色学 (HPLC) 在40分钟的时间内分离乙烯 Ester.
使用高分辨率质谱法 (HRMS) 进行识别和量化.

主要成果:

成功识别和定量各种乙烯 Ester 的物种.
证明了基于HRMS的方法的高度敏感性和特异性.
验证了在生物样本 (包括肝脏恒星细胞) 中分析乙烯的方法.

结论:

高分辨率质谱仪为敏感的网乙烯分析提供了强大的工具.
开发的方法提供了对视网体代谢的改进特征.
这种技术对研究与视网相关的生物过程具有重大意义.