FBXW7基因的表观遗传状态及其在卵巢癌发病过程中的作用
Meerah Al Hinai1, Shika Hanif Malgundkar1, Ishita Gupta2
1Sultan Qaboos University Hospital, Oman.
Asian Pacific journal of cancer prevention : APJCP
|May 29, 2023
概括
FBXW7基因表达在上皮卵巢癌 (EOC) 中降低. 无论是突变还是甲基化都不能解释这一点,但长非编码RNA LINC01588 可能起着调节作用.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 表皮卵巢癌 (EOC) 病原包括潜在的候选人,如FBXW7基因和LINC01588.
- 在EOC中FBXW7和LINC01588的确切作用仍然不清楚.
- 这项研究调查了FBXW7突变和EOC中的甲基化状态的影响.
研究的目的:
- 确定FBXW7突变/甲基化与其在EOC中的表达之间的相关性.
- 探索FBXW7基因与EOC中的LINC01588表达之间的关系.
- 在EOC中阐明FBXW7的监管机制.
主要方法:
- 利用公共数据库对FBXW7突变/甲基化和表达的相关性分析.
- 执行了皮尔森的相关性分析,以评估FBXW7和LINC01588.8之间的关系.
- 经过验证的生物信息学发现,使用EOC细胞系和患者样本的基因组外体序列和甲基化特异PCR (MSP).
主要成果:
- 在EOC组织中,FBXW7基因表达显著降低,特别是在晚期 (III和IV).
- 在EOC细胞系和组织中没有检测到FBXW7基因的突变或甲基化.
- 在FBXW7和LINC01588表达之间观察到一个反向和显著的相关性,表明LINC01588.8.的潜在调节作用.
结论:
- 在EOC中FBXW7的下调不是由突变或甲基化引起的.
- 为FBXW7.7提出了可能涉及长非编码RNA LINC01588的替代性调节机制.
- 通过与FBXW7表达的反相关性,LINC01588可能在上皮卵巢癌中发挥调节作用.
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