伤口愈合期间单细胞和细胞因子的动态变化烧伤后伤害伤害
Lindy Schaffrick1, Jie Ding1, Peter Kwan2
1Wound Healing Research Group, Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada.
Cytokine
|May 29, 2023
概括
烧伤伤害可以导致高缩性痕 (HTS). 这项研究发现特定的单细胞变化和增加的细胞因子 (IL-6,IL-8,RANTES,MCP-1) 与烧伤严重程度相关,为HTS发展提供了见解.
科学领域:
- 免疫学 免疫学 免疫学
- 伤口治愈研究研究 伤口治愈研究
- 皮肤病学 皮肤病学
背景情况:
- 在全球范围内,烧伤伤害使许多人面临高风险的发展高缩性痕 (HTS).
- 过度缩性痕的特点是疼痛,起和收缩的纤维组织,影响移动性和美学.
- 了解燃烧损伤的系统反应对于预防和治疗HTS至关重要.
研究的目的:
- 调查单细胞亚群和细胞因子的作用,在伤口愈合过程中,烧伤后的伤害.
- 为了将这些免疫反应与烧伤伤害的严重程度以及随后的缩性痕的发展相关联.
- 确定新型治疗策略的潜在目标,以预防和治疗缩性痕.
主要方法:
- 招募了27名烧伤患者和13名健康对照人群,根据烧伤总体表面积 (TBSA) 对患者进行分层.
- 分析了血清和外周血液单核细胞 (PBMCs),使用酶相关免疫吸收试验 (ELISAs) 检测细胞因子 (IL-6,IL-8,IL1RA,IL-10) 和化学因子通路 (SDF-1/CXCR4,MCP-1/CCR2,RANTES/CCR5).
- 利用流细胞计量来评估单细胞亚群和化学因受体表达,通过ANOVA和Pearson的相关性进行统计分析.
主要成果:
- 在受伤4-7天后发展HTS的患者中,CD14+CD16单细胞亚群更大.
- 烧伤损伤增加了CD14+CD16+单细胞上的CXCR4,CCR2和CCR5表达.
- 提高IL-6,IL-8,RANTES和MCP-1的水平与烧伤严重程度的增加有显著的相关性.
结论:
- 单细胞动态和化学因受体表达在烧伤后发生变化,可能导致缩性痕形成.
- 系统性细胞因子水平,包括IL-6,IL-8,RANTES和MCP-1,与烧伤严重程度有正相关性.
- 进一步评估单细胞,化学因受体和细胞因子对于理解异常伤口愈合和开发有效的HTS治疗方法至关重要.
相关概念视频
Phases of Wound Repair
6.1K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
6.1K
Inflammatory Response II: Inflammatory Exudate and Tissue Repair
5.2K
The immune system's inflammatory response destroys the invading pathogen, permitting the tissue to heal. The changes during the cellular and vascular stages allow exudate formation at the site of inflammation. The inflammatory exudate released from the wound has high protein content and a specific gravity above 1.020.
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
5.2K
Inflammatory Response I: Vascular and Cellular
11.5K
The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
11.5K
Inflammatory Response
2.2K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
2.2K
Inflammation
54.0K
Overview
54.0K
Differentiation of Common Myeloid Progenitor Cells
3.3K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.3K


