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Ps^-基于Pt纳米酶的协同信号放大生物传感器用于高度敏感的蛋白质颜色测量检测

Chaohui Chen¹, Mengting Qi², Cheng Fu¹

¹State Key Laboratory of Precision Blasting, Jianghan University, Wuhan 430056, PR China; Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, College of Photoelectric Materials and Technology, Jianghan University, Wuhan 430056, Hubei, China.

|May 29, 2023

概括

这项研究引入了一种新的免疫色度测定生物传感器,用于敏感的α-fetoprotein (AFP) 检测. 新方法提高了AFP的灵敏度和视觉检测极限,改进了传统的酶相关免疫吸收试验 (ELISA).

科学领域:

生物医学工程生物医学工程
分析化学分析化学
生物化学生物化学

背景情况:

酶相关免疫吸收试验 (ELISA) 是一种常见的阿尔法-胎蛋白 (AFP) 检测方法.
传统的ELISA在灵敏度上有局限性,原因是颜色测量信号较弱.
需要改进的检测方法来准确诊断AFP.

研究的目的:

开发一种高度敏感的免疫色度测量生物传感器,用于AFP检测.
为了克服传统ELISA的灵敏度限制.
创建一个快速和可视观察到的AFP检测系统.

主要方法:

开发了一种新的生物传感器,结合了Ps-Pt纳米酶和终端脱氧核酸转移酶 (TdT) 介导的聚合.
使用3,3',5,5'-四甲丁 (TMB) 与Ps-Pt和胡卜过氧化酶 (HRP) 的催化氧化进行色度检测.
测量视觉色彩强度用于AFP的确定.

主要成果:

生物传感器显示,对于AFP度在10-500 pg/mL之间,在25秒内出现显著的颜色变化.
达到了AFP的4.30 pg/mL的低检测极限.
在10 pg/mL的AFP显示了明显的视觉区分,并且适用于复杂的样本.

结论:

开发的免疫色度测量生物传感器提供了增强的灵敏度和快速检测AFP.
这种方法提供了视觉检测能力,改进了传统的ELISA.
生物传感器平台显示了在复杂的生物样本中检测其他蛋白质生物标记物的潜力.

关键词:

颜值测量颜色测量方法颜色测量方法蛋白质检测检测蛋白质检测检测这就是Ps-Ptt.协同效应的放大放大.

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相关概念视频

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...