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一种新型的His-tag结合性阿胺酶用于重组蛋白检测和基于T细胞的免疫疗法

Li-Ting Yang¹, Tuersunayi Abudureheman¹, Wei-Wei Zheng¹

¹Key Laboratory of Pediatric Hematology and Oncology Ministry of Health and Pediatric Translational Medicine Institute, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

|May 29, 2023

概括

研究人员使用分子信标选了一种新型的aptamer来检测使用His标记的蛋白质. 这种阿帕特马还使双特异的圆形阿帕特马能够用于增强的基于T细胞的癌症免疫疗法,显示出有前途的抗瘤疗效.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
免疫治疗是一种免疫疗法.

背景情况:

在抗体药物生产中,aptamers对于检测重组蛋白至关重要.
双特异的圆形吸收体通过吸引多种细胞类型,为向癌症治疗提供了潜力.
六西丁标签 (His标签) 在重组蛋白净化和检测中很常见.

研究的目的:

为了映和描述一本小说他的标签-绑定aptamer (20S).
开发一种分子信标 (MB) 用于敏感检测His标记蛋白质.
为基于T细胞的针对癌细胞的免疫疗法构建双特异性圆形体 (bc-apts).

主要方法:

选一种高亲和度的His标签结合的阿普坦胺.
开发一种基于20S的分子信标 (20S-MB) 用于蛋白质检测.
通过用Sgc8 (PTK7-向性体) 循环化His-tag体构建bc-apts.
对针对瘤细胞的T细胞介导细胞毒性的阿巴胺-抗体复合物的评估.

主要成果:

阿普塔默20S对他的标签有很高的亲和力.
20S-MB在体外和体内都显示出对His标记蛋白质的敏感和特定检测,与ELISA一致.
两种特异性阿普坦 (20S-sgc8和6H5-sgc8) 被构建并与抗CD3抗体复合.
与6H5-sgc8相比,20S-sgc8胺-抗体复合体通过增强T细胞细胞毒性,表现出更高的抗瘤疗效.

结论:

一种新的His-tag-binding aptamer被确定并用于开发一种敏感的分子信标测试.
双特异性圆形体为增强基于T细胞的免疫疗法提供了可行的策略.
这项研究为复合蛋白检测和向癌症治疗提供了有前途的方法.

关键词:

双特异的圆形体体.他的标签结合的阿帕特默体.他的标记蛋白质.分子信标是一个分子信标.基于T细胞的免疫疗法.

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