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佩奥尼佛洛林通过通过KLF4介导的线粒细胞吸收来调节巨分化,抑制脏炎症

Yiwen Cao¹, Jingli Xiong¹, Xueping Guan¹

¹Department of Pharmacology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, China.

Phytomedicine : international journal of phytotherapy and phytopharmacology

|May 29, 2023

概括

佩奥尼佛林 (PF) 通过将M1巨细胞转移到M2并通过KLF4调节增强线粒细胞衰变来抑制慢性病 (CKD) 中的炎. 这为CKD治疗提供了潜在的新疗法策略.

科学领域:

腎臟病學 (nephrology) 是一種醫學.
免疫学免疫学免疫学
药理学药理学是指药理学的学科.

背景情况:

巨细胞M1极化是炎损伤和慢性病 (CKD) 的关键因素.
来自Paeonia lactiflora的Paeoniflorin (PF) 已显示出脏的抗炎性质,但其在CKD中的机制尚不清楚.

研究的目的:

在慢性病 (CKD) 的背景下,研究Paeoniflorin (PF) 对巨细胞两极分化的调节作用.

主要方法:

一个CKD小鼠模型被诱导使用cationic牛血清白蛋白.
用脂多糖 (LPS) 刺激小鼠巨细胞系RAW264.7,以模仿炎症状况.
分析了PF对巨细胞极化,功能,病理性损伤,线粒体功能和与线粒体相关的蛋白质的影响.

主要成果:

通过抑制M1巨标记物 (CD68,iNOS) 和增加M2标记物 (CD206),PF改善了功能,并减少了CKD小鼠的病理损伤.
在体外,PF降低了促炎性细胞因子 (IL-6,IL-1β,TNF-α,MCP-1) 和增加了抗炎性标记物 (Arg1,Fizz1,IL-10,Ym-1),表明M1到M2两极分化.
通过调节克鲁佩尔样因子4 (KLF4),PF增强了线粒体功能,降低了氧化应激,并通过调节克鲁佩尔样因子4 (KLF4) 调节了与线粒细胞衰变相关的蛋白质 (PINK1,帕金,Bnip3,P62,LC3).

结论:

佩奥尼佛洛林 (PF) 通过促进M1到M2巨细胞的两极分化,并通过KLF4诱导线粒细胞衰变来抑制CKD中的炎.
PF显示出作为治疗慢性病 (CKD) 的新疗法战略的潜力.

关键词:

慢性脏疾病慢性脏疾病克鲁佩尔类转录因子4 巨细胞的两极分化线粒细胞衰变 - - 线粒细胞衰变 (mitophagy) 是一种佩奥尼弗洛林是一种.

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