相关实验视频

Updated: Jul 28, 2025

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

用胰腺癌的无细胞DNA进行突变分析

Yorihiko Takase¹, Yoshiki Naito², Akihiko Kawahara¹

¹Department of Diagnostic Pathology, Kurume University Hospital, Kurume, Japan.

Anticancer research

|May 29, 2023

概括

从胰腺癌细胞学样本中检测细胞自由DNA中的KRAS突变有助于差异诊断. 这种方法显示出作为识别胰腺癌的辅助工具的希望.

科学领域:

在瘤学瘤学.
分子诊断学分子诊断学
胃肠病学胃肠病学

背景情况:

胰腺癌的检测是具有挑战性的小内镜超声波引导细针吸收 (EUS-FNA) 样本.
准确的差异诊断对于有效的胰腺癌治疗至关重要.

研究的目的:

评估从液体细胞学 (LBC) 超级生物中在无细胞DNA (cfDNA) 中检测KRAS突变,作为胰腺癌的辅助诊断测试.
为了比较cfDNA KRAS突变状态与甲固定嵌 (FFPE) 组织的结果.

主要方法:

一项对50名胰腺病变患者的队列研究.
将cfDNA从固定LBC样本的浮物中分离出来.
在cfDNA和FFPE组织之间对KRAS突变状态的比较.

主要成果:

84% (42/50) 的cfDNA样本可用于分析.
在57.1% (24/42) 的有效cfDNA样本中检测到KRAS突变.
使用cfDNA检测KRAS突变的灵敏度,特异性和准确性分别为63.2%,100.0%和66.7%.

结论:

来自固定LBC超标的cfDNA的基因突变分析是胰腺癌诊断的有效辅助工具.
这种方法为改善胰腺病变的诊断准确性提供了一种有希望的方法.

关键词:

在KRAS突变中发生突变.胰腺癌是一种胰腺癌.细胞学无细胞DNA DNA 内镜超声波引导细针吸收细胞学.基于液体的细胞学.

更多相关视频

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines

Published on: October 8, 2015

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

相关概念视频

The Ras Gene

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...