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通过ROS介导的TCA循环与Bacillus thuringiensis的紫外线抵抗有很大关系

Xi Du¹, Yile Zhang¹, Haonan Wu¹

¹State Key Laboratory of Ecological Pest Control for Fujian, Taiwan Crops & Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education & Ministerial, Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, College of Plant Protection and College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Pesticide biochemistry and physiology

|May 29, 2023

概括

提灵菌 (Bt) 生物杀虫剂对紫外线辐射的耐药性涉及减少的三碳酸 (TCA) 循环. 这种代谢转变降低了活性氧物种 (ROS) 的产生,增强了Bt.

科学领域:

微生物学微生物学
生物化学生物化学
环境科学环境科学

背景情况:

菌 (Bt) 是一种环保的生物杀虫剂,由于对紫外线辐射的敏感性而面临限制.
紫外线暴露显著降低了Bt的有效性和持久性,需要制定改善其环境稳定的策略.

研究的目的:

阐明Bt对紫外线辐射的抗性背后的代谢机制.
为了比较野生型Bt和抗紫外线突变的代谢特征.

主要方法:

对Bt LLP29和抗紫外线突变LLP29-M19.9的代谢分析进行比较.
对差异性代谢物丰度和代谢途径的丰富性进行分析.
RT-qPCR用于评估TCA循环和活性氧物种 (ROS) 途径的基因表达.
酶活性测定关键的TCA循环酶.

主要成果:

确定了61种不同丰富的代谢物,在12个途径中显著丰富,包括TCA循环.
关键的TCA循环基因和酶活性 (ICDH,SDH,PDH) 在抗UV突变者中显著下调.
抑制TCA循环增强了紫外线抵抗力,并降低了ROS生成途径的调节.

结论:

降低TCA循环活性对于增强Bacillus thuringiensis抗紫外线辐射的作用至关重要.
降低ROS生成通路的调节有助于UV电阻,由TCA循环调节.
代谢重编程提供了一种可行的策略,以提高Bt生物农药的紫外线抵抗力.

关键词:

甲状腺杆菌 (Bt) 甲状腺杆菌 (Bt)代谢学代谢学代谢学这就是ROSOS ROS.在TCA循环中,TCA循环的周期是:抗紫外线的抗 UV 射线的抗性.

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