比较的生理和转录组分析提供了关于6-pentyl-2H-pyran-2-one对Clarireedia jacksonii的抑制作用的见解
Man Liu1, Qichen Niu1, Ziyue Wang1
1School of Grassland Science, Beijing Forestry University, Beijing 100083, China.
Pesticide biochemistry and physiology
|May 29, 2023
概括
这项研究揭示了6-pentyl-2H-pyran-2-one (6PP) 如何通过破坏细胞膜和改变代谢途径来对抗草美元斑点真菌. 这些发现支持6PPPP.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
- 生物化学 生物化学
背景情况:
- 克拉里雷迪亚spp. 克拉里雷迪亚sp. 在曲草地上造成破坏性的草.
- 6--2-H-pyran-2-one (6PP) 是一种具有生物控制潜力的天然代谢物.
- 6PP对 Clarireedia jacksonii 的抗真菌机制尚未完全理解.
研究的目的:
- 为了阐明6PP的抗真菌机制对抗Clarireedia jacksonii.
- 为了研究C. jacksonii对6PP暴露的生理和分子反应.
- 为使用6PP作为绿色农药提供理论基础.
主要方法:
- 用6PP.治疗C. jacksonii的方法.
- 检测细胞膜完整性,活性氧物种 (ROS),麦隆迪化物 (MDA),酒精脱酶 (ADH) 和乙甲脱酶 (ALDH).
- 使用基因本体学和基因和基因组的京都百科全书 (KEGG) 的基因表达分析.
主要成果:
- 6PP抑制了C. jacksonii菌株的生长,损害了细胞膜的完整性.
- 6PP增加了ROS和MDA水平,改变了ADH/ALDH活性,并激活了抗氧化酶.
- 细胞壁/膜完整性和氧化应激基因的下调;脂肪酸降解,ATP合成,ABC载体和谷氨代谢基因的上调.
结论:
- 6PP通过破坏细胞膜并诱导氧化应激,表现出抗真菌活性.
- 脂肪酸降解和ATP合成的升级可能会促进真菌的亡.
- 基因表达的变化表明对6PP的复杂反应,突出了其作为新型生物杀虫剂的潜力.
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