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Updated: Jul 28, 2025

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在山 (Dioscorea sp.) 中艾伦托因的量化. 使用一个H NMR光谱方法
Thao Quyen Cao1, Dongyup Hahn1,2,3
1Institute of Agricultural Science and Technology, Kyungpook National University, Daegu 41566, Republic of Korea.
Journal of microbiology and biotechnology
|May 29, 2023
概括
一种新的定量核磁共振 (qNMR) 方法准确地测量了山中的艾伦因,这是已知的皮肤保护剂. 这一进步克服了先前用于确定艾伦因的HPLC方法的局限性.
科学领域:
- 分析化学 分析化学
- 自然产品化学 自然产品化学
背景情况:
- 在中丰富地发现的艾伦因具有皮肤保护性药理作用.
- 之前的艾伦因测定方法,如高性能液态染色学 (HPLC),由于分离不够令人满意.
- 准确量化艾伦因对于了解其益处和应用至关重要.
研究的目的:
- 开发和验证一种新的定量核磁共振 (qNMR) 方法,用于在中确定艾伦.
- 与现有技术相比,提高艾伦因量化的准确性和可靠性.
主要方法:
- 开发一种1H定量核磁共振 (qNMR) 方法.
- 作为内部标准,利用了阿兰因和二甲基硫 (DMSO2) 之间的相对信号整合比率.
- 方法验证包括特异性,线性 (62.52000微克/毫升),灵敏度 (LOD 4.63微克/毫升,LOQ 14.03微克/毫升),精度 (RSD% 0.020.26) 和回收率 (86.3592.11%).
主要成果:
- 成功建立了一种经过验证的1H qNMR方法来定量艾伦因.
- 该方法表现出极好的线性,灵敏度,精度和回收.
- 开发的方法用于量化四种不同的韩国山品种的肉和皮的提取物中的艾伦因.
结论:
- 开发的1H qNMR方法为山中的艾伦因确定提供了优质的替代方案,解决了先前HPLC技术的局限性.
- 这种经过验证的方法可以准确地评估各种子部分和品种中的艾伦因含量.
- 这些发现有助于更好地了解和利用来自自然来源的艾伦因,如用于护肤应用的.
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