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Updated: Jul 28, 2025

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
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Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

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通过基于物理模型的背景过算法进行定量结构化照明显微镜,揭示了actin动态

Yanquan Mo1, Kunhao Wang2, Liuju Li1

  • 1State Key Laboratory of Membrane Biology, Center for Life Sciences, College of Future Technology, Peking University, Beijing, 100871, China.

Nature communications
|May 29, 2023
PubMed
概括

我们开发了一种新的方法,SIM的背景过 (BF-SIM),以改进活细胞超分辨率显微镜. 这种技术增强了微妙结构的成像,并保持了新发现的信号精度.

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Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles08:02

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相关概念视频

Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
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