提取物Trametes versicolor (火尾) 的抗毒素活动
Homa Nath Sharma1,2,3, Jonathan Catrett4, Ogechi Destiny Nwokeocha5,6
1Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA.
Scientific reports
|May 29, 2023
概括
土耳其尾提取物在体外有效抑制了Toxoplasma gondii tachyzoites的生长. 这种天然化合物显示出作为治疗毒素菌的安全和负担得起的替代方案的希望.
科学领域:
- 菌类学 菌类学是指菌类学.
- 寄生虫学的寄生虫学
- 药理学 药理学是指药理学的学科.
背景情况:
- 毒素菌感染由于患病率上升,造成了全球重大健康挑战.
- 毒素菌现有的治疗方法受到有效性,安全性和成本的限制所阻碍.
研究的目的:
- 为了研究更高的真菌提取物对抗Toxoplasma gondii的疗效.
- 探索火尾提取物对T. gondii的潜在作用机制.
- 评估提取物在宿主细胞上的安全性.
主要方法:
- 土耳其尾 (TT) 的甲醇提取物在体外对T. gondii tachyzoites (RH-RFP菌株) 进行了测试.
- 使用光板阅读器测量了塔基佐伊特生长抑制.
- 评估了宿主细胞 (人类前皮纤维细胞) 的生存能力.
- 分析了线粒体活动和膜潜力.
- 扫描电子显微镜被用来观察形态变化.
主要成果:
- TT提取物显著抑制了T. gondii tachyzoites的生长,其IC50为5.98±1.22μg/mL.
- 提取物对人类前皮纤维细胞具有较低的细胞毒性,CC50≥100μg/mL.
- TT提取物诱导了线粒体超氧化物和反应性氧物种的产生,破坏了寄生虫的线粒体膜潜力.
- 在接受了TT提取物和pyrimethamine治疗后,观察到tachyzoites的形态变形.
结论:
- 土耳其尾甲醇提取物,富含植物,脂,,酸和乳,是新型抗毒素菌剂的潜在来源.
- 提取物的机制涉及到T. gondii tachyzoites中线粒体功能的破坏.
- TT提取物代表了一个有希望的,非细胞毒性的候选人,用于开发新的毒素菌治疗方法.
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